结核蛋白芯片对肺结核诊断的临床价值

中国防痨杂志 ›› 2005, Vol. 27 ›› Issue (6): 367-369.

结核蛋白芯片对肺结核诊断的临床价值

郭光云¹；陈功¹；郭凌郧²；王家宁²；

1.湖北省郧阳医学院附属人民医院十堰 442000；2.郧阳医学院附属人民医院临床医学研究所十堰 442000；

出版日期:2005-06-10 发布日期:2005-11-03

Clinical value of tuberculosis protein chip in diagnosis of pulmonary tuberculosis

Guo Guangyun,Chen Gong,Guo lingyun,et al.

Respiratory Department of People’s Hospital Affiliated to YunYang Medical College,ShiYan 442000,China

Online:2005-06-10 Published:2005-11-03

摘要/Abstract

摘要： 目的　探讨结核蛋白芯片、痰涂片抗酸染色及结核菌素(PPD)皮试单独和联合检测对肺结核患者临床诊断的意义。方法对45例肺结核和30例非结核性肺疾病患者,同时进行结核蛋白芯片、痰抗酸杆菌直接涂片、PPD皮试,观察单项和联合指标对诊断肺结核的敏感性与特异性。结果结核蛋白芯片、PPD及痰涂片的敏感性分别为66.7%、44.4%、22.2%,特异性分别为93.3%、84.6%、100%。结核蛋白芯片联合PPD对肺结核的阳性检出率为86.7%,结核蛋白芯片联合PPD及痰涂片的阳性检出率提高到88.9%,与单项检测的最高阳性检出率相比差异有显著性意义(P<0.05)。三种方法联合检测的特异性为80%,与PPD或结核蛋白芯片单项检测相比无显著性差异(P>0.05)。结论结核蛋白芯片是诊断结核病较有效方法,具有快速方便等优点,蛋白芯片联合PPD、痰涂片抗酸染色,能显著提高肺结核的阳性检出率,但特异性并没有明显下降。

关键词: 结核诊断,肺, 蛋白质序列分析

Abstract: Objective To study the diagnostic value of tuberculosis protein chip,acid-fast smear and tuberculin purified protein derivative(PPD) in pulmonary tuberculosis. Methods 45 patients with active pulmonary tuberculosis,30 patients with respiratory system diseases other than tuberculosis as control were tested by tuberculosis protein chips,acid-fast smear and PPD,respectively.The Results were compared with each other. Results The examination sensitivitives of tuberculosis protein chip,PPD and acid-fast smear were 66.7%,44.4%,22.2% respectively,and the specificity was 93.3%,84.6%,100 %.The detection rate of combined method was obviously higher than that of the single test,and the positive rates increased by 88.9%.But he specificity remain 80%. Conclusion Tuberculosis protein chip may be used To test Mycobacterium tuberculosis quickly and accurately.The combination had an increased detection rate in detecting pulmonary tuberculosis and remain a higher specificity.

Key words: Pulmonary tuberculosis/diagnosis, Protein sequence analysis

郭光云,陈功,郭凌郧,王家宁,. 结核蛋白芯片对肺结核诊断的临床价值[J]. 中国防痨杂志, 2005, 27(6): 367-369.

Guo Guangyun,Chen Gong,Guo lingyun,et al.. Clinical value of tuberculosis protein chip in diagnosis of pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2005, 27(6): 367-369.