延边州结核分枝杆菌DNA指纹图谱特征分析

中国防痨杂志 ›› 2005, Vol. 27 ›› Issue (1): 23-25.

延边州结核分枝杆菌DNA指纹图谱特征分析

李书琳¹；金德福²；韩中波³；田花子²；矫庆辉³；张永胜³；

1.吉林市卫生局吉林 132011；2.吉林延边结核病防治所延边 133000；3.吉林市结核病防治研究所吉林 132011；

出版日期:2005-01-10 发布日期:2005-11-03
基金资助:
吉林省卫生厅医学科研资助 2000-143。

The analysis of characteristics of DNA fingerprint patterns of Mycobacterium tuberculosis strains in Yanyi City

Li Shulin,Jin Defu,Han Zhongbo,et al.

Jilin City Tuberculosis Institute,Jilin City 132011,China

Online:2005-01-10 Published:2005-11-03

摘要/Abstract

摘要： 目的　建立延边州结核分枝杆菌IS6110 RFLP指纹图谱 ,并分析朝鲜、汉两族的图谱特征。方法 97株结核分枝杆菌分离株 ,提取DNA ,经酶切 ,电泳 ,Southem转印 ,探针杂交 ,发光自显影技术得到IS6110 RFLP图谱；按其指纹图谱特征的同源性高低进行分析。结果延边州结核分枝杆菌IS6110拷贝数目平均为 13个；57.7%菌株可成簇 ,具有“北京基因型”特征；IS6110 RFLP图谱特征分布无地域差异 ,无民族差异。结论延边州结核分枝杆菌分离株同北京分离株有较近的亲缘关系 ,但其多态性较高。

关键词: 分枝杆菌,结核, 指纹图谱, 延边州

Abstract: Objective To establish IS6110 RFLP fingerprint patterns of Mycobacterium tuberculosis isolated from Yanji City and analyze the national characteristics of Han and Korean nationalities.Methods To abstract the DNA from 97 mycobacterium tuberculosis strains,using endonuclease,southern blot,probe hybridization and chemiluminescence to get IS6110 RFLP fingerprint patterns,analyze the characteristics according to their homology of IS6110 RFLP fingerprint.Results The characteristics of IS6110 RFLP patterns of mycobacterium tuberculosis strains isolated from Yanji City showed:(1)The average of the copies of IS6110 is 13.(2)The clustering rate of strains was 57.7%,they had the characteristics of “Beijing genotype strains".(3)The characteristics of IS6110 RFLP fingerprint patterns were not related to the geographic and ethnic distribution of the patterns.Conclusion The isolated strains of Mycobacterium tuberculosis from Yanji City was closely relative to “Beijing genotype strains”,but there was a feature specially,that was the high polymorphism.

Key words: Mycobacterium tuberculosis, Fingerprint patterns, Yanji City

李书琳,金德福,韩中波,田花子,矫庆辉,张永胜,. 延边州结核分枝杆菌DNA指纹图谱特征分析[J]. 中国防痨杂志, 2005, 27(1): 23-25.

Li Shulin,Jin Defu,Han Zhongbo,et al.. The analysis of characteristics of DNA fingerprint patterns of Mycobacterium tuberculosis strains in Yanyi City[J]. Chinese Journal of Antituberculosis, 2005, 27(1): 23-25.

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