分支杆菌分子菌种鉴定方法的研究进展

摘要/Abstract

摘要： 由于全球范围内肺结核及非结核分支杆菌病的发病呈逐年上升趋势,准确而快速的菌种鉴定技术对结核病的诊断和鉴别诊断及有效化疗都有极其重要的意义。目前国内外采用传统的分支杆菌菌种鉴定方法需依据细菌生长速度、色素产生及多种生化试验等结果综合分析才能获得结果;而ＢＡＣＴＥＣＴＢ460、ＴＢ960培养基系统虽快速(2～6ｄ),但只能初步鉴定结核分支杆菌复合群和非结核分支杆菌,不能将细菌鉴定至种。近年来建立的色谱方法虽能达到部分菌种鉴定的目的,但需特殊仪器,临床尚未普遍开展。目前国内外研究者都致力于使分支杆菌菌种鉴定从细胞表型水平进入分子基因水平。现对分支杆菌分子菌种鉴定方法的研究进展综述如下。

梁建琴,吴雪琼,张广宇,. 分支杆菌分子菌种鉴定方法的研究进展[J]. 中国防痨杂志, 2002, 24(4): 227-230.

参考文献

[1] Portillo P D,Murillo L A,Patarroyo M E,et al.Amplification of a species specific DNA fragment of M.tuberculosis and its possible use in diagnosis[J].J Clin Microbiol,1991,29(10):2163～2168.
[2] Patel R J,Fries J W U,Piessens W F,et al.Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for indetification of M .tuberculosis[J].J Clin Microbiol,1990,28(3):513～518.
[3] Wit D D,Steyn L,Shoemaker S,et al.Direct detection of M.tuberculosis in clinical specimens by DNA amplification[J].J Clin Microbiol,1990,28(11):2437～2441.
[4] Roth A,Fischer M,Hamid M E,et al.Differentiation of phyloge-netically related slowly growing Mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences[J].J Clin Microbiol,1998,36(1):139～147.
[5] Soini H,Botter E C,Viljanen M K.Identification of Mycobacteria by PCR-based sequence determination of the 32-kilodalton protein gene[J].J Clin Microbiol, 1994,32(12):2944～2947.
[6] Shoemaker S A,Fisher J H,Scoggin C H.Techniques of DNA hybridization detect small numbers of Mycobacteria with no cross-hybridization with non mycobacterial respiratory organisms.Am Rev Respir Dis,1985,131:760.
[7] 吴雪琼，庄玉辉，黄蓉蓉，等.人型结核杆菌全染色体DNA探针鉴别结核杆菌和其它分支杆菌的研究[J].微生物学报，1990,30(3):234～237.
[8] Takewaki S I,Okuzumi K,Ishiko H,et al.Genus-specific poly-merase chain reaction for the Mycobacterial dnaj gene and spe-sies-specific oligonuleotide probes[J].J clin Microbiol,1993,31(2):446～450.
[9] Kox L F F,Leeuwen J V,Knijper S,et al.PCR assay based on DNA coding for 16S RNA for detection and indetification of My-cobacteria in clinical samples[J].J Clin Microbiol,1995,33(12):3225～3233.
[10] Patel S,Yaates M,Saunders N A.PCR-enzyme-linked immu-noscorbent assay and partial rRN A gene sequencing:a rational approach to identify Mycobacteria [J].J Clin M icrobiol,1995,35(9):2375～2380.
[11] Vaneechoutte M,De Beenhouuer H,Claeys G,et al.Identification of mycobacterium species by using amplified ribosomal DNA re-striction analysis [J].J Clin Microbiol, 1993,31(8):2061～2065.
[12] Avaniss-Aghajan E,Jones K,Holtzman A,et al.Molecular tech-nique for rapid identification of mycobacteria[J].J Clin Microbiol, 1996,34:98～102.
[13] Plikaytis B B,Plikaytis B D,Yakrus M A,et al.Differentiation of slowly growing Mycobacterium species,including mycobacterim tuberculosis,by gene amplification and restriction fragment length polymorphism analysis[J].J Clin Microbiol,1992,30(7):1815～1822.
[14] Devallois A,Goh K S,Rastogi N.Rapid identification of Myco-bacteria to species level by PCR-restriction fragment length poly-morphism analysis of the hsp65 gene and proposition of analgo-rithm to differentiate 34 mycobacterial species[J].J Clin Microbiol,1997,35:2969～2673.
[15] Lappayawichit P,Rienthong S,Rienthong D,et al.Differentiation between Mycobacterium species by restriction enzyme analysis of amplified 16S-23S ribosomal DNA spacer sequences[J].Tubercle Lung Dis,1996,77:257～263.
[16] Sansila A,Hongmanee P,Chuchotlaworn C,et al.Differentiation between Mycobaclerium tuberculosis and Mycobacterium avium by amplification of the 16S-23S ribosomal DNA spacer[J].J Clin Microbiol,1998,36(9):2399～2403.
[17] Takewaki S,Okuzumi K,Manade I,et al.Nucleotide sequence comparison of the mycobacterial danj gene and PCR-restriction fragment length polymorphism analysis for identification of my-cobacterial species[J].Int J Syst Bacteriol,1994,44:159～166.
[18] 吴雪琼，王晋洪，张俊仙，等.PCR-SSCP分支杆菌菌种初步鉴定方法的建立及其应用[J].中国现代医学杂志，2000,10(7):25～26.
[19] Gingeras T R,Ghandour G,Wang E,et al.Simultaneous geno-typing and species identification using hybridization pattern rec-ognition analysis of Mycobacterium DNA arrays[J].Genome Re-search,1998,8:435～448.
[20] Troesch A,Nguyen H,Miyada C G,et al.Mycobacterium species identification and rifampin resistance testing with high-density DNA probe arrays[J].J Clin Microbiol, 1999,37(1):49～55.