联检脑脊液中LAM-IgG和TB-DNA在诊断结核性脑膜炎中的应用

中国防痨杂志 ›› 2001, Vol. 23 ›› Issue (5): 284-285.

联检脑脊液中LAM-IgG和TB-DNA在诊断结核性脑膜炎中的应用

薛承岩；刘兰芳；刘霞；刘李承；米艳娟；

河北省承德医学院附属医院承德 067000；

出版日期:2001-05-10 发布日期:2001-11-03

Uniting detection of lipoarabinomannan-IgG and TB-DNA in cerebrospinal fluid for the diagnosis of tuberculous meningitis

XUE Cheng-yan,LIU Lan-fang,LIU Xia,et al.

Affiliated Hospital of Chengde Medical College,Chengde 067000

Online:2001-05-10 Published:2001-11-03

摘要/Abstract

摘要： 目的　了解脑脊液 (CSF)中阿拉伯糖甘露糖脂IgG抗体 (LAM-IgG)和TB-DNA指标对结脑的诊断价值。方法以CSF为标本，用酶联免疫吸附试验 (ELISA)检测LAM-IgG，用聚合酶链反应(PCR)检测TB-DNA。结果 102份结脑病人CSF标本，LAM-IgG阳性率51.0% (52102)，TB-DNA阳性率81.4% (83102)，LAM-IgG阳性及或TB-DNA阳性共93例 (91.2%)。40份非结核性的中枢神经系统疾病病例的CSF标本，均未检出LAM-IgG和TB-DNA.结论 LAM-IgG和TB-DNA均是诊断结脑的较好的指标，两者联检可进一步提高检测敏感性。

关键词: 脑膜炎,结核性, 脑脊髓液, 阿拉伯糖甘露糖脂-IgG, TB-DNA

Abstract: Objective To understand the value of lipoarabinomannan-IgG and TB-DNA in cerebrospinal fluid (CSF) in the diagnosis of tuberculous meningitis.Methods CSF was the detective specimen.LAM-IgG was detected with enzyme linked immunosorbent assay (ELISA).TB-DNA was detected with polymerase chain reaction (PCR).Results The positive rate of LAM-IgG and TB-DNA in 102 patients with tuberculous meningitis was 51.0%(52/102) and 81.4%(83/102).The positive rate of LAM-IgG and TB-DNA was 91.2%(93/102).But 40 control cases were all negative.Conclusion LAM-IgG and TB-DNA in CSF were useful index for the diagnosis of tuberculous meningitis.

Key words: Meningitis,tuberculosis, Cerebrospinal fluid, Lipoarabinomannan-IgG, TB-DNA

薛承岩,刘兰芳,刘霞,刘李承,米艳娟,. 联检脑脊液中LAM-IgG和TB-DNA在诊断结核性脑膜炎中的应用[J]. 中国防痨杂志, 2001, 23(5): 284-285.

XUE Cheng-yan,LIU Lan-fang,LIU Xia,et al.. Uniting detection of lipoarabinomannan-IgG and TB-DNA in cerebrospinal fluid for the diagnosis of tuberculous meningitis[J]. Chinese Journal of Antituberculosis, 2001, 23(5): 284-285.

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