关键词: 结核,骨关节, HLA-DR.DQ基因, 聚合酶链反应-序列特异性

Abstract: Objective To study the association with HLA-DR.DQ genes in these people with Tuberculosis of the bone and joint. Susceptible gene was compared Tuberculosis of the bone and joint with pulmonary Tuberculosis. Methods The alleles at the HLA-DR.DQ typing were analyzed by polymerase chain reaction with sequence-specific primers(PCR-SSP)in 86 patients with tuberculosis of the bone and joint,34 patients with pulmonary tuberculosis and 88 healthy controls.Results Compared tuberculosis of the bone and joint with normal people,A significant increased frequency of DRB1*09 and DQB1*0301 was found in tuberculosis of the bone and joint(38.99% vs 8.24% PC<0.0001 RR=8.92;18.27% vs 2.66% PC<0.05 RR=2.21).The freuqency of DRB1*13.2 in tuberculosis of the bone and joint was significantly decreased(4.76% vs 20.50% PC<0.01 RR=0.18).Compared tuberculosis of the bone and joint with pulmonary tuberculosis,A significant increased frequency of DRB1*09 and DQB1*0301 was found in tuberculosis of the bone and joint(38.99% vs 9.2% PC<0.0001;18.15% vs 1.85% PC<0.01).The frequency of DRB1*15 in tuberculosis of the bone and joint was significantly decreased(17.17% vs 36.3% PC<0.01).Conclusion The Results showed that HLA-DRB1*09 and DQB1*0301 were probably susceptible gene in tuberculosis of the bone and joint,HLA-DRB1*13.2 may be a protective gene.It was different for susceptible gene between tuberculosis of the bone and joint and pulmonary tuberculosis.

Key words: Tuberculosis,osteoarticular, HLA-DR.DQ alleles, PCR-SSP