Preliminary study on early diagnosis and efficacy evaluation of pulmonary tuberculosis based on molecular beacon real-time PCR

Abstract

Abstract: Objective To investigate early diagnosis and efficacy evaluation of pulmonary tuberculosis using molecular beacon real-time polymerase chain reaction(PCR). Methods Molecular beacon real-time PCR and acid-fast staining were used to detect Mycobacterium tuberculosis in 305 sputum specimens collected from pulmonary tuberculosis suspects at initial testing and 77 sputum specimens collected from pulmonary tuberculosis patients. Results Using acid-fast staining and molecular beacon real-time PCR, the positive rates were 12.6% (48/382), 42.4%（162/382）, respectively. The positive rates of detection of molecular beacon real-time PCR was significantly higher than that of acid-fast staining（χ²=74.5，P＜0.001). Conclusion Compared with acid-fast staining, molecular beacon real-time PCR shows higher sensitivity in detection of pulmonary tuberculosis. It enhances the detection rate and indirectly reflects efficacy of medications, thus showing promising in control and prevention of tuberculosis.

Key words: Tuberculosis,pulmonary/diagnosis, Tuberculosis,pulmonary/drug therapy, Polymerase chain reaction, Treatment outcome

CAO Ran-ran, LIU Bang-ying, ZHOU Zi-ren, GAO Wen-feng, YANG De-hui, YANG Hong, LIU Heng-chuan. Preliminary study on early diagnosis and efficacy evaluation of pulmonary tuberculosis based on molecular beacon real-time PCR[J]. Chinese Journal of Antituberculosis, 2012, 34(2): 103-106.

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