Serodiagnostic value of recombinant Mtb81 protein from M.tuberculosis for tuberculosis

Abstract

Abstract: Objective To evaluate the value of recombinant Mtb81 (rMTB81) protein from M.tuberculosis on the serodiagnosis of tuberculosis (TB), and to develop more effective diagnostic reagents for TB. Methods The rMTB81 protein was expressed and purified by metal chelation chromatography. The antibody IgG against M.tuberculosis rMtb81 antigen in 402 human sera were detected by ELISA. Results The rMTB81 protein existed in inclusion bodies of E. coli, and amounted to 30% of total bacterial proteins. Its molecular weigh...

Key words: serologic tests, Mycobacterium tuberculosis, recombinant proteins, bacterial proteins, antigens, bacterial

CLC Number:

R446.6

Yang Yourong,Wu Xueqiong,Zhang Junxian,Liang Yan,Zhang Cuiying,Dong Mei,Chen Hongbing,He Jufang. Serodiagnostic value of recombinant Mtb81 protein from M.tuberculosis for tuberculosis[J]. Chinese Journal of Antituberculosis, 2010, 32(7): 38-41.

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