Abstract: ObjectiveTo study the clinical value of fast detection acid-fast bacilli by centrifuging the liquefied sputa and collecting bacteria. MethodsCompared the positive rate of outpatient sputum samples which detected by centrifuging the liquefied sputa and collecting bacteria with that by conventional smear method simultaneously, and evaluated this method in comparison with L-J medium used to detect the sputum samples of inpatients. ResultsThe positive rate of 240 outpatient sputum samples by centrifuging the liquefied sputa and collecting bacteria and by conventional smear method were 40.8% and 25.8%, respectively. There was significant difference. Using L-J medium as the control, the sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 90.9%, 93.8%, 97.6%, 78.9% and 91.7% respectively. ConclusionsThe centrifuging the liquefied sputa and collecting bacteria is a clinical testing method, which is easy to be standardized, simplified and accurated.

Key words: Tuberculosis,pulmonary/diagnosis, Mycobacterium tuberculosis, Colony count,microbial