Evaluation of antigenicity of 5 Mycobacterium tuberculosis specific proteins

Abstract

Abstract: ObjectiveTo evaluate antigenicity of 5 Mycobacterium tuberculosis specific proteins including 14, 16, 38 kD, Mtb81 and ESAT-6. MethodsAll the recombinant proteins of 14, 16, 38 kD, mtb81 and ESAT-6 were generated, and employed in ELISA to detect the antibodies in serum samples of 120 TB patients, 30 non-TB patients and 30 normal human. ResultsThe Results showed that all 5 proteins gave above 95% specificity, while 38 kD showed 80.8% sensitivity which was more sensitive than any other assays using 14, 16 kD, Mtb81 and ESAT-6 recombinant proteins. ConclusionAll 5 specific proteins of Mycobacterium tuberculosis showed different levels of antigenicity. It was useful for TB diagnosis, and improved assay might be helpful to detect the antigens or antibodies against them.

Key words: Mycobacterium tuberculosis, Recombinant proterins, Antigens,bacterial, Bacterial proteins

Yang Liu,Su Mingquan, Yue Qiaohong, Zhang Jianfang, Ma Yueyun,Liu Jiayun, Hao Xiaoke. Evaluation of antigenicity of 5 Mycobacterium tuberculosis specific proteins[J]. Chinese Journal of Antituberculosis, 2009, 31(7): 381-383.

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