Identification of strains isolated from tuberculosis outbreak in a high school

Abstract

Abstract: ObjectiveTo identify the bacterial species and strain types of isolates from tuberculosis outbreak in a high school. MethodsFrom May to August 2006, one teacher and 64 students developed clinical symptoms of active tuberculosis in a high school, Liaoning Province. Clinical isolates from sputa were collected and identified by phenotypic tests; conventional biochemical methods; amplification of hupB gene, dnaA-dnaN region, NTF-1 region, Spoligotyping and micobacterial interspersed repetitive unit (MIRU) typing; partial sequencing of 16S rRNA, 16S-23S internal transcribed spacer (ITS) region, hsp65 gene (441 bp) and PCR restriction enzyme analysis of the hsp65 gene. ResultsClinical isolates were identified as Mycobacterium tuberculosis complex and non-tuberculous mycobacteria (NTM) by conventional biochemical methods. Subsequent analyzed by phenotypic tests and amplification of hupB gene, dnaA-dnaN region, NTF-1 region, Spoligotyping and MIRU genotyping, these Results indicated that the species identification of mycobacterium tuberculosis complex as mycobacterium tuberculosis Beijing genotype modern strain, the genotype profile of MIRU was 223325173533. By partial sequencing of 16S rRNA, 16S-23S ITS region, hsp65 gene (441 bp) and PCR restriction enzyme analysis of the hsp65 gene supported the species identification of MOTT as M. porcinum. ConclusionThe mycobacterium species isolated from patients involved in tuberculosis outbreak are mycobacterium tuberculosis Beijing genotype modern strain and M. porcinum, the significance of M.porcinum in tuberculosis outbreak should be further studied.

Key words: tuberculosis/epidemiology, disease outbreaks, Mycobacterium tuberculosis, mycobacteria, atypical, bacteriological techniques

Wang Shengfen¹, Wang Li², Yang Lijun²,Jiang Guanglu¹, Xia Hui¹, Zhou Yang¹, Shang Mei, Li Qiang¹, Zhao Bing¹, Zhao Yanlin¹. Identification of strains isolated from tuberculosis outbreak in a high school[J]. Chinese Journal of Antituberculosis, 2009, 31(10): 573-578.

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