Detection of the mutations of katG,rpoB and embB Genes in Mycobacterium tuberculosis from sputum specimens with PCR-SSCP technique

Abstract

Abstract: Objective To detect the mutations of katG,rpoB and embB genes in Mycobacterium tuberculosis(MTB) from sputum specimens by PCR-SSCP method,and to evaluate the clinical value of this method.Methods 110 MTB isolates from sputum specimens were detected by PCR-SSCP and conventional drug susceptibility testing.The sequences of katG,rpoB and embB were also analyzed.Results The sensitivities of mutations of katG,rpoB and embB genes analyzed by PCR-SSCP were 55.9%,76.0% and 46.4%,respectively.Their specificities were 70.0%,90.0% and 60.0%,respectively.Conclusions PCR-SSCP technique is a rapid,simple,high sensitive and strongly specific method for detection of mutations of katG,rpoB and embB genes in MTB from sputum specimens.

Key words: Mycobacterium tuberculosis, Mutation, PCR

Zhu Min¹,Li Feng,Sheng Guoping,et al.. Detection of the mutations of katG,rpoB and embB Genes in Mycobacterium tuberculosis from sputum specimens with PCR-SSCP technique[J]. Chinese Journal of Antituberculosis, 2007, 29(6): 508-510.

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