The study of rpoB gene mutation of Mycobacterium tuberculosis

Abstract

Abstract: Objective To investigate the characteristics of rpoB gene mutation of Mycobacterium tuberculosis in local area. Methods 65 Mycobacterium tuberculosis strains were isolated.The rpoB genes containing codon 509 to codon 631 were detected with PCR-SSCP and gene sequencing. Results The mutation rate of rpoB gene from RFP-resistant strains was 96.4%(27/28),which contained 64.3%(18/28)mutation in codon 526,21.4%(6/28)in codon 513,7.1%(2/28)in codon 531,3.6%(1/28)in codon 529.The mutation rate of rpoB gene from RFP-sensitive but other drugs-resistant strains was 18.5%(5/27)and the mutation site was irregular.None mutation was found in drug-sensitive strains. Conclusion The mutation of rpoB is correlative with RFP resistance.Codon 526 and codon 513 are the most frequent in local area.rpoB mutation is also found in other drug-resistant strains.

Key words: Mycobacterium tuberculosis, rpoB gene, Gene mutation

Li Fujian¹,Li Feng,Li Jum.. The study of rpoB gene mutation of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2006, 28(5): 285-287.

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