RpoB gene mutations of rifampin-resistant Mycobacterium tuberculosis in Shandong Province

Abstract

Abstract: Objective To investigate gene mutations in the rifampin(RIF) resistance determining region(RRDR) of rpoB gene in Mycobacterium tuberculosis(MTB) isolates from Shandong Province,China.Method60 RIF-resistant and 75 RIF?susceptible MTB isolates collected from over 30 counties of Shandong Province in 1997,2000,and 2003 were analyzed by direct DNA sequencing.Results Fifty five(91.7%) of 60 RIF-resistant isolates were found missense mutations in RRDR of rpoB gene.The most common mutations occurred at codon 531(60.0%),526(29.1%) and 516(7.3%) respectively.One isolate presented multiple mutations at codons 511,515,518 simultaneously.Only 3(4.0%) of 75 RIF-susceptible isolates showed mutations at codon 533 with one novel mutation type(CTG→ATG).Conclusion The agreement rate between RRDR mutations of rpoB gene and RIF resistance among 135 MTB isolates from Shandong Province is 94.1%(127/135).Mutations at codon 533 were not associated with RIF resistance.

Key words: Mycobacterium tuberculosis, rpoB gene, DNA direct sequencing

Wang haiying¹,Liu Zhimin,Xu Yong,et al.. RpoB gene mutations of rifampin-resistant Mycobacterium tuberculosis in Shandong Province[J]. Chinese Journal of Antituberculosis, 2005, 27(5): 289-292.

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