Abstract: Objective To identify the tubercle bacillus DNA with PCR microplate hybridization-ELISA. Methods To use PCR technology to expand tubercle bacillus DNA,and add the expanded matter into microplate with tubercle bacillus probes,then add tubercle bacillus probes of color displaying,and conduct color displaying of ELISA of microplate hybirdizationg.510 patients with pulmonary disease were tested.Results The sensitivity of the method is 59.3%,and its specificity is 95.0%.Positive predictive value is 96.3%,negative predictive value is 51.4%.Conclusion PCR microplate hybirdizatin-ELISA can be used to test tubercle bacillus DNA quickly and accurately,it is a reliable laboratory method early to diagnose and differentiate tuberculosis.

Key words: PCR, Hybridization, ELISA, Mycobacterium tuberculosis, DNA