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中国防痨杂志 ›› 2013, Vol. 35 ›› Issue (4): 273-275.

• 论著 • 上一篇    下一篇

四县（区）结核病防治机构固体培养能力建设调查

欧喜超 林百丰 胡培磊 夏辉 赵雁林   

  1. 102206 北京，中国疾病预防控制中心结核病预防控制中心国家结核病参比实验室（欧喜超、夏辉、赵雁林）；黑龙江省结核病防治所参比实验室（林百丰）；湖南省结核病防治所检验科（胡培磊）
  • 收稿日期:2013-01-15 出版日期:2013-04-10 发布日期:2013-07-02
  • 通信作者: 赵雁林 E-mail:zhaoyanlin@chinatb.org
  • 基金资助:

    卫生部-比尔和梅琳达·盖茨基金会结核病防治项目（2009-04-01）

Investigation on the ability of Mycobacterium solid culture in four county (district) tuberculosis dispensaries

OU Xi-chao, LIN Bai-feng, HU Pei-lei, XIA Hui, ZHAO Yan-lin   

  1. National Tuberculosis Reference Laboratory, National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention，Beijing 102206, China
  • Received:2013-01-15 Online:2013-04-10 Published:2013-07-02
  • Contact: ZHAO Yan-lin E-mail:zhaoyanlin@chinatb.org

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摘要： 目的  评估固体培养试验在我国县（区）级结核病防治机构（简称“结防机构”）应用的可行性。方法  选取黑龙江省北林区和兰西县及湖南省湘潭县和岳阳县4个县（区）级结核病实验室，自2010年5月至2011年2月连续纳入2059例具有肺结核可疑症状的初诊患者，收集患者痰标本完成分枝杆菌涂片镜检和固体培养试验，按月统计涂阳培阴率和污染率。按照培养试验室内质量控制要求，初诊患者涂阳培阴率要求低于10%，污染率要求控制在2%～5%。 结果  （1）全部患者涂片和培养阳性率分别为18.3%（376/2059）和26.6%（547/2059），差异有统计学意义（χ2=129.2，P<0.01）。（2）北林区涂阳培阴率为16.5%(23/139)，超过正常要求低于10%的水平。湘潭县和岳阳县培养污染率分别为5.6%(157/2800) 和9.7%(252/2590)，超过正常要求低于5%的水平。其余县（区）涂阳培阴率和培养污染率均处于正常范围。 结论 4个县（区）级实验室初步具备了分枝杆菌固体培养试验能力。

关键词: 分枝杆菌, 结核, 细菌学技术, 痰, 标本制备, 培养技术, 专业能力

Abstract: Objective To evaluate the feasibility of Mycobacterium solid culture in county (district) tuberculosis (TB) dispensaries. Methods  2059 doubtful patients with pulmonary TB from Xiangtan, Yueyang, Beilin and Lanxi county were continuously recruited from May 2010 to February 2011. The sputum samples from patients recruited were collected and performed smear examination and solid culture. The smear-positive and culture-negative rate and culture pollution rate were calculated each month.  Results (1) The smear-positive rate and culture-positive rate in all patients recruited were 18.3% (376/2059) and 26.6% (547/2059)，respectively. They had statistically significant difference (χ2=129.2, P<0.01) (2) The smear-positive and culture-negative rate in Beilin was 16.5% (23/139), higher than normal level. The rate of culture pollution in Xiangtan and Yueyang were 5.6% (157/2800) and 9.7%(252/2590)，respectively, both higher than normal level. The smear-positive and culture-ne-gative rates and culture pollution rates in other counties (district) were in normal range.  Conclusion Four laborato-ries in county (district) TB dispensaries have the primary ability on performing Mycobacterium solid culture.

Key words: Mycobacteriun tuberculosis, Bacteriological techniques, Sputum, Specimen handling, Culture techniques, Professional competence

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