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中国防痨杂志 ›› 2011, Vol. 33 ›› Issue (11): 758-761.

• 论著 • 上一篇    下一篇

荧光染色发光二极管荧光显微镜痰涂片镜检方法成本分析

夏辉 欧喜超 李强 赵冰 宋媛媛 王胜芬 赵雁林   

  1. 102206 北京，中国疾病预防控制中心结核病预防控制中心 国家结核病参比实验室
  • 收稿日期:2011-06-25 出版日期:2011-11-10 发布日期:2012-01-20
  • 通信作者: 赵雁林 E-mail:zhaoyanlin@chinatb.org
  • 基金资助:

    中国卫生部与比尔及梅琳达·盖茨基金会结核病防治项目（2009-04-01）

Cost analysis of smear microscopy using fluorescent staining and light-emitting diode fluorescence microscope

XIA Hui, OU Xi-chao, LI Qiang, ZHAO Bing, SONG Yuan-yuan, WANG Sheng-fen, ZHAO Yan-lin   

  1. National Tuberculosis Reference Laboratory, National Center for Tuberculosis Control and Prevention, China CDC, Beijing 102206, China
  • Received:2011-06-25 Online:2011-11-10 Published:2012-01-20
  • Contact: ZHAO Yan-lin E-mail:zhaoyanlin@chinatb.org

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摘要： 目的  对比分析萋尔-尼尔逊涂片镜检方法（简称"Z-N”）与荧光染色发光二极管荧光显微镜（LED）涂片镜检2种方法的实验室成本。  方法  收集6个县（区）级结核实验室使用Z-N和LED进行痰涂片镜检的直接和间接投入，比较分析6个实验室分别使用2种方法进行3次涂片镜检的平均成本。两样本间均数差别显著性检验采用t检验，多样本间均数差别显著性检验采用F检验，P<0.05为差异有统计学意义。  结果  Z-N法和LED方法检测1份痰标本的成本分别为人民币（14.98±3.94）元和（13.27±4.83）元(t=5.084, P=0.001)。6个实验室采用Z-N法进行1个痰涂片镜检的成本范围为人民币9.44～17.82元，LED法进行1个痰涂片镜检的成本范围为人民币7.80～18.93元。结论  LED进行痰涂片镜检的成本比Z-N法低，从成本角度LED更值得推广。

关键词: 显微镜检查, 荧光, 痰, 成本及成本分析

Abstract: Objective  To compare laboratory cost of smear microscopy using Ziehl-Neelsen(Z-N) method and fluorescent staining and light-emitting diode fluorescence microscope (LED) method.  Methods  Laboratory direct and indirect cost related to smear microscopy examination were collected from six county laboratories, based on 3 times examination, comparison of the average cost of smear microscopy between two methods was made.  t test and F test was performed to assess the significance of the difference between the means of two samples  and multi-samples. The P <0.05 is considered statistical significance.  Results  The mean cost of a smear examination with Z-N and LED were RMB (14.98±3.94) Yuan and (13.27±4.83) Yuan respectively (t=5.084, P=0.001). The range of cost of 6 laboratories is RMB 9.44－17.82 Yuan for Z-N  and 7.80－18.93 Yuan  for LED.  Conclusion  Cost of a smear examination with LED is lower than that with Z-N, therefore worth of promotion from cost of view. 

Key words: Microscopy,fluorescence, Sputum, Costs and cost analysis

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