The profile of gene expression from macrophage THP-1 infected by Mycobacterium bovis

Abstract

Abstract: ObjectiveTo select the candidate target for immunological diagnosis of bovine tuberculosis, and to study the immunological pathogenetic mechanisms of bovine tuberculosis caused by Mycobacterium bovis. MethodsThe gene expression profiles from macrophage THP-1 infected by Mycobacterium bovis (AF2212/97) and Mycobacterium tuberculosis (H37Rv) 72 hours later were analyzed with gene chips containing 22000 full-length genes. The protein expression level and their interaction of corresponding gene were compared and analyzed by GO and Pathway significant statistical analysis. ResultsThere were expression difference of 290 and 1899 genes in macrophage THP-1 cells infected by Mycobacterium tuberculosis and Mycobacterium bovis, respectively. These differential genes mainly included inflammatory factors, apoptosis proteins, extracellular matrix protein, the proteins correlating with T-cell receptor signaling pathway, and other related proteins by GO and Pathway analysis. ConclusionsThe result of gene expression profile could provide the useful biology information for the diagnostic and pathogenetic study of bovine tuberculosis.

Key words: tuberculosis bovis, macrophages, cytokines, gene expression profiling

Gao Feng¹，Li Weimin²，Li Chuanyou²，Liu Yi², Sun Zhaogang²,Zhang Jianyuan²,Jiang Ping¹,Fan Weixing³. The profile of gene expression from macrophage THP-1 infected by Mycobacterium bovis[J]. Chinese Journal of Antituberculosis, 2009, 31(3): 150-156.

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