The diagnostic value of simultaneous amplification and testing for Mycobacterium tuberculosis technique on alveolar lavage fluid for diagnosing pulmonary tuberculosis

doi:10.19982/j.issn.1000-6621.20230006

Abstract

Abstract:

Alveolar lavage fluid specimens from inpatients with clinical diagnosis of suspected tuberculosis in Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment from October 2021 to July 2022 were collected, and acid-fast bacilli smear, mycobacterium culture (MGIT 960) and strain identification, GeneXpert MTB/RIF and simultaneous amplification and testing for Mycobacterium tuberculosis (SAT-TB) test were performed. The results were analyzed and evaluated. Taking culture result (MGIT 960) as the standard, the sensitivity of SAT-TB in the diagnosis of tuberculosis was 80.61% (399/495), and the specificity was 87.74% (1259/1435), the positive predictive value was 69.39% (399/575), the negative predictive value was 92.92% (1259/1355), and the Youden index was 0.68. SAT-TB technique has high sensitivity and specificity, is of certain value for rapid diagnosis of active tuberculosis in clinic practice.

Key words: Mycobacterium tuberculosis, Nucleic acid amplification techniques, Molecular diagnostic techniques

CLC Number:

R521

Zou Yuanwu, Li Jing, Wang Biao, Wang Zhuo, Qu Shaoyi, Yuan Zhimin, Wu Qianhong. The diagnostic value of simultaneous amplification and testing for Mycobacterium tuberculosis technique on alveolar lavage fluid for diagnosing pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2023, 45(4): 434-436. doi: 10.19982/j.issn.1000-6621.20230006

Figures/Tables 1

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检测方法	结核分枝杆菌培养		敏感度 (%,95%CI值)	特异度 (%,95%CI值)	阳性预测值 (%,95%CI值)	阴性预测值 (%,95%CI值)	约登指数 (95%CI值)	Kappa值 (95%CI值)
检测方法	阳性	阴性	敏感度 (%,95%CI值)	特异度 (%,95%CI值)	阳性预测值 (%,95%CI值)	阴性预测值 (%,95%CI值)	约登指数 (95%CI值)	Kappa值 (95%CI值)
SAT-TB			80.61 (0.76~0.84)	87.74 (0.86~0.89)	69.39 (0.65~0.73)	92.92 (0.92~0.95)	0.68 (0.65~0.73)	0.57 (0.53~0.61)
阳性	399	176
阴性	96	1259
GeneXpert MTB/RIF			97.58 (0.95~0.97)	88.78 (0.88~0.90)	75.00 (0.71~0.78)	99.07 (0.98~0.99)	0.86 (0.84~0.90)	0.64 (0.60~0.68)
阳性	483	161
阴性	12	1274
涂片			47.88 (0.43~0.52)	96.24 (0.95~0.97)	81.44 (0.76~0.85)	84.26 (0.83~0.86)	0.44 (0.38~0.50)	0.35 (0.31~0.40)
阳性	237	54
阴性	258	1381