结核病临床诊治进展年度报告（2011年）

摘要/Abstract

摘要： 结核病临床诊断和治疗的历史源远流长。在人类社会与结核病斗争的历史长河里，取得的每一次关键性进步都是以结核病诊断和治疗技术方面的重大进展为标志的。从100多年以前德国科学家罗伯特·科赫发现了Mtb，到以后结核菌素及细菌学、影像学和分子生物学检测技术方面所取得的成就，为结核病的临床诊断注入了更大的活力；从1944年S的发明，到INH、对氨基水杨酸（PAS）、EMB、氨硫脲(Thz)以及RFP等的相继问世，使结核病的临床治疗迈入了一个崭新的时代，从而有效地控制了结核病的流行与传播。然而，目前全球结核病的疫情十分严重，结核病仍然是威胁人类健康的主要疾病之一。作为中国防痨协会分支机构的结核病临床专业委员会致力于通过开展学术活动和服务性工作增强协会的影响力和凝聚力，积极推动结核病的规范化诊断、治疗，通过多种形式的活动提高学术水平。为及时了解、掌握国内外结核病临床诊治的新观点、新技术、新进展及发展趋势，进一步提高广大医务工作者对结核病临床工作的认识，增进同行在结核病临床诊治研究领域的交流、普及，推广国内外有关结核病的临床诊治的最新进展，结核病临床专业委员会在2011年开始计划试行编纂《年度结核病临床诊治技术进展报告》制度。于每年年末到次年年初，通过收集本年度国内外有关结核病临床诊治技术进展的信息，组织专家加以荟萃、分析、推荐和解读，形成一份具有参考价值的学术资料。

中国防痨协会临床专业委员会. 结核病临床诊治进展年度报告（2011年）[J]. 中国防痨杂志, 2012, 34(6): 393-403.

. Annual report on clinical diagnosis and treatment progress of tuberculosis (2011)[J]. Chinese Journal of Antituberculosis, 2012, 34(6): 393-403.

参考文献

［1］Minion J, Pai M, Ramsay A, et al. Comparison of LED and conventional fluorescence microscopy for detection of acid fast bacilli in a low-incidence setting. PLoS One, 2011, 6(7):e22495.

［2］姜广路, 黄海荣, 赵雁林, 等. 痰涂片阳性结核病患者的涂片与培养结果分析. 中华结核和呼吸杂志, 2011, 34(5): 353-355.

［3］张娟, 蒋俊, 张红, 等. MGIT960与罗氏培养法在结核分枝杆菌培养及药敏试验中的比对分析. 中国防痨杂志, 2011, 33（6）: 361-365.

［4］Lu PL,Yang YC, Huang SC, et al. Evaluation of the Bactec MGIT 960 system in combination with the MGIT TBc identification test for detection of Mycobacterium tuberculosis complex in respiratory specimens. J Clin Microbiol, 2011, 49(6):2290-2292.

［5］Said HM, Ismail N, Osman A, et al. Evaluation of TBc identification immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in samples from broth cultures. J Clin Microbiol, 2011，49(5):1939-1942.

［6］Zhu C, Cui Z, Zheng R, et al. A multi-center study to evaluate the performance of phage amplified biologically assay for detecting TB in sputum in the pulmonary TB patients. PLoS One, 2011, 6(9):e24435.

［7］谭耀驹, 蔡杏珊, 吴幸怡, 等. 噬菌体生物扩增法检测痰液结核分枝杆菌的临床应用. 中华检验医学杂志, 2011, 34(5): 448-450.

［8］Mi X, He F, Xiang M, et al. Novel phage amplified multichannel series piezoelectric quartz crystal sensor for rapid and sensitive detection of Mycobacterium tuberculosis. Anal Chem, 2012, 84(2): 939-946.

［9］Dusthackeer VN, Balaji S, Gomathi NS, et al. Diagnostic luciferase reporter phage assay for active and non-replicating persistors to detect tubercle bacilli from sputum samples. Clin Microbiol Infect, 2012, 18(5):492-496.

［10］Leung E, Minion J, Benedetti A, et al. Microcolony culture techniques for tuberculosis diagnosis: a systematic review. Int J Tuberc Lung Dis, 2012, 16(1):16-23.

［11］Comina G, Mendoza D, Velazco A, et al. Development of an automated MODS plate reader to detect early growth of Mycobacterium tuberculosis. J Microsc, 2011, 242(3): 325-330.

［12］武洁, 桂晓虹, 李静, 等. 比例法与绝对浓度法检测结核分枝杆菌药敏试验的比较. 中华检验医学杂志, 2011, 34(2):137-138.

［13］李静, 桂晓红, 孙丕, 等. MGIT液体培养基检测分枝杆菌效果的评价. 中华检验医学杂志, 2011, 34(2): 111-114.

［14］陆俊梅, 王洁, 黄晓辰, 等. 显微镜观察药物敏感性检测技术在痰标本直接药敏试验中的应用. 中华预防医学杂志, 2011, 45(1): 21-24.

［15］谭守勇, 谭耀驹, 陈松, 等. 噬菌体生物扩增法检测涂片阳性痰标本中结核分枝杆菌对利福平的耐药性. 中华传染病杂志, 2011, 29(3): 164-167.

［16］李同心, 崔振玲, 汪小黎, 等. 噬菌体生物扩增法检测结核分枝杆菌阿米卡星耐药性方法的建立及应用评价. 中国防痨杂志, 2011, 33(3): 158-162.

［17］Bwanga F, Haile M, Joloba ML, et al. Direct nitrate reductase assay versus microscopic observation drug susceptibility test for rapiddetection of MDR-TB in Uganda. PLoS One, 2011, 6(5): e19565.

［18］黄海荣, 于霞, 姜广路, 等. 利奈唑胺对分枝杆菌体外抑菌作用的初步研究.中华结核和呼吸杂志, 2011, 34(8):575-578.

［19］桂徐蔚, 肖和平, 胡忠义, 等. 氯法齐明对不同耐药类型结核分枝杆菌的体外抑菌活性研究. 中华结核和呼吸杂志, 2011, 34(8): 579-581.

［20］Karsen H, Koruka ST, Karahocagil MK, et al. Comparative analysis of cerebrospinal fluid. Swiss Med Wkly, 2011,141:w13214.

［21］Sun Q, Sha W, Xiao HP, et al. Evaluation of cerebrospinal fluid adenosine deaminase activity for the differential diagnosis of tuberculous and nontuberculous meningitis. Am J Med Sci, 2011 ［Epub ahead of print］.

［22］闫世明, 秦桂香, 赵云虹, 等. 结核性脑膜炎脑脊液细胞学的临床研究.中国防痨杂志, 2011, 33（12）：780-784.

［23］Ozsahin SL, Arslan S, Epozturk K, et al. Chest X-ray and bacteriology in the initial phase of treatment of 800 male patients with pulmonary tuberculosis. J Bras Pneumol, 2011, 37(3): 294-301.

［24］Agizew T, Bachhuber MA, Nyirenda S, et al. Association of chest radiographic abnormalities with tuberculosis disease in asympomatic HIV-infected adults. Int J Tuberc Lung Dis, 2010, 14(3): 324-331.

［25］Eisenberg RL, Pollock NR. Low yield of chest radiography in a large tuberculosis screening program. Radiology, 2010, 256(3): 998-1004.

［26］Kowada A, Deshpande GA, Takahashi O, et al. Cost-effectiveness analysis of interferon-r release assays versus chest X-ray for annual tuberculosis screening of healthcare workers. J Hosp Infect, 2011, 78(2):152-154.

［27］陆普选. 结核病对临床影像学诊断的挑战. 放射学实践, 2011, 26（9）：916-917.

［28］Yeh JJ, Yu JK, Teng WB, et al. High-resolution CT for identify patients with smear-positive, active pulmonary tuberculosis. Eur J Radiol, 2012, 81(1): 195-201.

［29］Yeh JJ, Chen SC, Teng, WB, et al. Identifying the infectious lesions in pulmonary tuberculosis by high-resolution muti-detector computed tomography. Eur Radiol, 2010, 20(9): 2135-2145.

［30］陈殿森, 关文华, 张璇. 低剂量CT扫描在评价老年菌阴性肺结核患者抗结核治疗效果中的应用. 中华老年医学杂志, 2011, 30（8）: 626-628.

［31］Luo MY, Liu L, Lai LS, et al. Deepgoing study on intrathoracic tuberculous lymphadenitis in adults using multidetector CT. Chin Med J (Engl), 2010, 123(10): 1283-1288.

［32］Mukund A, Khurana R, Bhalla AS, et al. CT patterns of nodal disease in pediatric chest tuberculosis. World J Radiol, 2011, 3(1): 17-23.

［33］郑斐群, 刘厚明, 李玮, 等. 脓肿型分枝杆菌肺部感染胸片和CT表现. 放射学实践, 2011, 26(9): 928-930.

［34］Polverosi R, Guarise A, Balestro E, et al. High-resolution CT of nontuberculous mycobacteria pulmonary infection in immunocompetent, non-HIV-positive patients. Radiol Med, 2010, 115（2）:191-204.

［35］朱文科, 陆普选, 乐晓华, 等. 艾滋病合并肺结核CT与病理对照分析. 放射学实践, 2011, 26 (9): 931-933.

［36］Kim JY, Jeong YJ, Kim KI, et al. Miliary tuberculosis: a comparison of CT findings in HIVseropositive and HIV-seronegative patients. Br J Radiol, 2010, 83(987):206-211.

［37］杨静, 马大庆, 张岩松, 等. 急性粟粒性肺结核的CT及病理对照研究. 中华放射学杂志, 2011, 45（6）: 520-523.

［38］Okamura K, Nagata N，Kumazoe H，et al. Relationship between computed tomography findings and nutritional status in elderly patients with pulmonary tuberculosis. Intern Med, 2011, 50(17): 1809-1814.

［39］Li Y, Su M, Li F, et al. The value of 18F-FDG-PET/CT in the differential diagnosis of solitary pulmonary nodules in areas with a high incidence of tuberculosis. Ann Nucl Med, 2011, 25(10):804-811.

［40］Kim DW, Kim CG. Dual-time point positron emission tomography findings of benign mediastinal lymph nodes in a tuberculosis-epidemic region. Jpn J Radiol, 2011, 29(10):682-687.

［41］Sathekge M, Buscombe JR. Can positron emission tomography work in the African tuberculosis epidemic? Nucl Med Commun, 2011, 32(4):241-244.

［42］Soussan M, Brillet PY, Mekinian A, et al. Patterns of pulmonary tuberculosis on FDG-PET/CT. Eur J Radiol, 2011 Sep 24 ［Epub ahead of print］.

［43］Zheng Z, Pan Y, Guo F, et al. Multimodality FDG PET/CT appearance of pulmonary tuberculoma mimicking lung cancer and pathologic correlation in a tuberculosis-endemic country. South Med J, 2011, 104(6): 440-445.

［44］Sathekge M, Maes A, Kgomo M, et al. Use of 18F-FDG PET to predict response to first-line tuberculostatics in HIV-associated tuberculosis. J Nucl Med, 2011, 52(6): 880-885.

［45］Razak HR, Geso M, Abdul Rahim N, et al. Imaging characteristics of extrapulmonary tuberculosis lesions on dual time point imaging (DTPI) of FDG PET/CT. J Med Imaging Radiat Oncol, 2011,55(6):556-562.

［46］Kosterink JG. Positron emission tomography in the diagnosis and treatment management of tuberculosis. Curr Pharm Des, 2011, 17(27), 2875-2880.

［47］揭冰, 江森, 孙希文, 等. 磁共振增强扫描后液体衰减反转恢复序列在结核性脑膜炎诊断中的应用.中华临床医师杂志电子版, 2011, 5（17）:5100-5103.

［48］Santy K, Nan P, Chantana Y, et al. The diagnosis of brain tuberculoma by 1H-magnetic resonance spectroscopy. Eur J Pediatr, 2011, 170(3):379-387.

［49］过丽芳, 周新华, 李宏军, 等. MR扩散加权成像在脑实质结核中的应用价值.放射学实践, 2011, 26(9): 918-922.

［50］Anuradha HK, Garg RK, Sinha MK, et al. Intracranial tuberculomas in patients with tuberculous meningitis: predictors and prognostic significance. Int J Tuberc Lung Dis, 2011, 15(2): 234-239.

［51］过丽芳, 周新华, 谢汝明, 等. 82例脑实质结核和肺癌脑转移瘤的MRI鉴别.中国防痨杂志, 2011, 33(12): 784-789.

［52］Dekker G, Andronikou S, van Toorn R, et al. MRI findings in children with tuberculous meningitis: a comparison of HIV-infected and non-infected patients. Childs Nerv Syst, 2011, 27(11): 1943-1949.

［53］刘莹, 王云玲, 王红, 等. MR弥散加权成像在兔脊柱结核早期诊断中的应用价值. 磁共振成像, 2011,2(6):435-438.

［54］屈瑾, 雷新玮, 祁吉. 早期不典型脊椎结核和化脓性脊椎炎的MR鉴别诊断. 中国医学影像技术, 2010, 26(2): 323-326.

［55］Walsh MC, Camerlin AJ, Miles R, et al. The sensitivity of interferon-γ release assays is not compromised in tuberculosis patients with diabetes. Int J Tuberc Lung Dis, 2011, 15(2): 179-184.

［56］Hang NT, Lien LT, Kobayashi N, et al. Analysis of factors lowering sensitivity of interferon-γ release assay for tuberculosis. PLoS One, 2011, 6(8): e23806.

［57］Lee YJ, Lee J, Kim YY, et al. Performance of whole-blood interferon-gamma release assay in patients admitted to the emergency department with pulmonary infiltrates. BMC Infect Dis, 2011, 11:107.

［58］Fujita A, Ajisawa A, Harada N, et al. Performance of a whole-blood interferon-gamma release assay with mycobacterium RD1-specific antigens among HIV-infected persons. Clin Dev Immunol, 2011, 2011. pii: 325295.

［59］World Health Organization. Use of Interferon-γ release assays (IGRAs) in tuberculosis control in low and middle-income settings: Expert group meeting report. HO/HTM/TB/2011.17. Geneva：WHO，2011. http://whqlibdoc.who.int/hq/2011/WHO_HTM_TB_2011-17_eng.pdf

［60］World Health Organization. Use of tuberculosis interferon-gamma release assays (IGRAs) in low-and middle-income countries: Policy Statement. WHO/HTM/TB/2011.18. Geneva：WHO，2011.http://whqlibdoc.who.int/publications/2011/9789241502672_eng.pdf

［61］Aabye MG, Ravn P, Johansen IS, et al. Incubation of whole blood at 39°C augments gamma interferon (IFN-γ) induced protein 10 and IFN-responses to Mycobacterium tuberculosis Antigens. Clin Vaccine Immunol, 2011, 18(7): 1150-1156.

［62］Ruhwald M, Dominguez J, Latorre I, et al. A multicentre evaluation of the accuracy and performance of IP-10 for the diagnosis of infection with M. tuberculosis. Tuberculosis (Edinb), 2011, 91(3):260-267.

［63］Lai CC, Tan CK, Lin SH, et al. Diagnostic performance of whole-blood interferon-γ assay and enzyme-linked immunospot assay for active tuberculosis’. Diagn Microbiol Infect Dis, 2011,71(2):139-143.

［64］Lee JE, Kim HJ, Lee SW. The clinical utility of tuberculin skin test and interferon-γ release assay in the diagnosis of active tuberculosis among young adults: a prospective observational study. BMC Infect Dis, 2011,11:96.

［65］Ra SW, Lyu J, Choi CM, et al. Distinguishing tuberculosis from Mycobacterium avium complex disease using an interferon-gamma release assay. Int J Tuberc Lung Dis, 2011,15(5):635-640.

［66］Cuccu B, Freer G, Genovesi A, et al. Identification of a human immunodominant T-cell epitope of Mycobacterium tuberculosis antigen PPE44. BMC Microbiology, 2011, 11:167.

［67］McNab FW, Berry MP, Graham CM, et al. Programmed death ligand 1 is over-expressed by neutrophils in the blood of patients with active tuberculosis. Eur J Immunol, 2011, 41(7): 1941-1947.

［68］Kasprowicz VO, Mitchell JE, Chetty S, et al. A molecular assay for sensitive detection of pathogen-specific T-cells. PLoS One, 2011, 6(8): e20606.

［69］Li L, Qiao D, Fu X, et al. Identification of M.tuberculosis specific Th1 cells expressing CD69 generated in vivo in pleural fluid cells from patients with tuberculous pleurisy. PLoS One, 2011, 6(8): e23700.

［70］Li L, Qiao D, Fu X, et al. Identification of Mycobacterium tuberculosis specific Th1, Th17 and Th22 cells using the expression of CD40L in tuberculosis pleurisy. PLoS One, 2011, 6(5): e20165.

［71］Dosanjh DP, Bakir M, Millington KA, et al. Novel M. tuberculosis antigen-specific T-cells are early markers of infection and disease progression. PLoS One, 2011, 6(12): e28754.

［72］Millington KA, Fortune SM, Low J, et al. Rv3615c is a highly immunodominant RD1 (Region of Difference 1) dependent secreted antigen specific for Mycobacterium tuberculosis infection. Proc Natl Acad Sci USA, 2011, 108(14): 5730-5735.

［73］Said Toihir AH, Richard V, Raharimanga V, et al. Evaluation of an immunochromatographic test for the diagnosis of pulmonary tuberculosis in Madagascar. BMC Research Notes, 2011, 4:403.

［74］Dowdy DW, Steingart KR, Pai M. Serological testing versus other strategies for diagnosis of active tuberculosis in India: a cost-effectiveness analysis. PLoS Med, 2011, 8(8): e1001074.

［75］Steingart KR, Flores LL, Dendukuri N, et al. Commercial serological tests for the diagnosis of active pulmonary and extrapulmonary tuberculosis: an updated systematic review and meta-analysis. PLoS Med, 2011, 8(8): e1001062.

［76］World Health Organization. World Health Organization(2011) policy statement: commercial serodiagnostic tests for diagnosis of tuberculosis. Geneva: WHO, 2011.

［77］Rekha RS, Kamal SM, Andersen P, et al. Validation of the ALS assay in adult patients with culture confirmed pulmonary tuberculosis. PLoS One, 2011, 6(1): e16425.

［78］Brust B, Lecoufle M, Tuaillon E, et al. Mycobacterium tuberculosis lipolytic enzymes as potential biomarkers for the diagnosis of active tuberculosis. PLoS One, 2011,6(9):e25078.

［79］何秀云, 黄香玉, 郝娟, 等.重组结核分枝杆菌PPE65蛋白的制备及其在肺结核诊断中的价值. 中华检验医学杂志, 2011, 34(7):633-637.

［80］范超明, 李伟, 樊德利, 等. 双抗原夹心胶体金免疫层析法快速检测结核分枝杆菌抗体的研究. 中华结核和呼吸杂志, 2011, 34（5）: 356-358.

［81］Patel VB, Singh R, Connolly C, et al. Comparative utility of cytokine levels and quantitative RD-1-specific T cell responses for rapid immunodiagnosis of tuberculous meningitis. J Clin Microbiol, 2011, 49(11): 3971-3976.

［82］Shao Y, Xia P, Zhu T, et al. Sensitivity and specificity of immunocytochemical staining of mycobacterial antigens in the cytoplasm of cerebrospinal fluid macrophages for diagnosing tuberculous meningitis. J Clin Microbiol, 2011, 49(9): 3388-3391.

［83］Vidhate MR, Singh MK, Garg RK, et al. Diagnostic and prognostic value of Mycobacterium tuberculosis complex specific interferon gamma release assay in patients with tuberculous meningitis. J Infect, 2011, 62(5): 400-403.

［84］Blakemore R, Nabeta P, Davidow AL, et al. A multisite assessment of the quantitative capabilities of the Xpert MTB/RIF assay. Am J Respir Crit Care Med, 2011,184(9):1076-1084.

［85］Teo J, Jureen R, Chiang D, et al. Comparison of two nucleic acid amplification assays, the Xpert MTB/RIF assay and the amplified Mycobacterium tuberculosis Direct assay, for detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J Clin Microbiol, 2011, 49(10): 3659-3662.

［86］Armand S, Vanhuls P, Delcroix G, et al. Comparison of the Xpert MTB/RIF test with an IS6110-TaqMan real-time PCR assay for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J Clin Microbiol, 2011, 49(5):1772-1776.

［87］Zeka AN, Tasbakan S, Cavusoglu C. Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens. J Clin Microbiol, 2011, 49(12): 4138-4141.

［88］Boehme CC, Nicol MP, Nabeta P, et al. Feasibility, diagnostic accuracy, and effectiveness of decentralised use of the Xpert MTB/RIF test for diagnosis of tuberculosis and multidrug resistance: a multicentre implementation study. Lancet, 2011, 377(9776):1495-1505.

［89］Miller MB, Popowitch EB, Backlund MG, et al. Performance of Xpert MTB/RIF RUO assay and IS6110 real-time PCR for Mycobacterium tuberculosis detection in clinical samples. J Clin Microbiol, 2011, 49(10): 3458-3462.

［90］Huang WL, Chi TL, Wu MH, et al. Performance assessment of the GenoType MTBDRsl test and DNA sequencing for detection of second-line and ethambutol drug resistance among patients infected with multidrug-resistant Mycobacterium tuberculosis. J Clin Microbiol, 2011, 49(7):2502-2508.

［91］García-Sierra N, Lacoma A, Prat C, et al. Pyrosequencing for rapid molecular detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis strains and clinical specimens. J Clin Microbiol, 2011, 49(10):3683-3686.

［92］Friedrich SO, Venter A, Kayigire XA, et al. Suitability of Xpert MTB/RIF and genotype MTBDR plus for patient selection for a tuberculosis clinical trial. J Clin Microbiol, 2011, 49(8): 2827-2831.

［93］刘申, 刘智勇, 黄庆, 等. 基因芯片检测系统在分枝杆菌属鉴定及耐药性分析的临床应用. 中华医院感染学杂志, 2011, 21(10): 1959-1962.

［94］周杨, 欧喜超, 乐军, 等. 基因芯片诊断耐多药结核病的临床多中心研究. 中华检验医学杂志, 2011, 34(9), 793-799.

［95］张俊仙, 吴雪琼, 阳幼荣, 等. 应用基因芯片方法检测结核分枝杆菌利福平和异烟肼的耐药性. 中国防痨杂志, 2011, 33（10）: 680-685.

［96］Bang H, Park S, Hwang J, et al. Improved rapid molecular diagnosis of multidrug-resistant tuberculosis using a new reverse hybridization assay, REBA MTB-MDR. J Med Microbiol, 2011, 60(Pt 10): 1447-1454.

［97］胡思玉, 牛建军, 权胜卯, 等. 探针熔解分析法检测结核分枝杆菌异烟肼耐药突变的应用和评价. 中华检验医学杂志, 2011, 34(10): 935-939.

［98］郑蓉蓉, 陈晓云, 付军, 等. 探针熔解分析法快速检测结核分枝杆菌乙胺丁醇耐药突变. 中华检验医学杂志, 2011, 34(2): 130-134.

［99］郑瑞娟, 秦莲花, 周燕, 等. 焦磷酸测序技术检测结核分枝杆菌四种药物耐药性. 中华检验医学杂志, 2011, 34(2): 115-120.

［100］周燕, 郑瑞娟, 秦莲花, 等. 焦磷酸测序技术快速检测结核分枝杆菌对氧氟沙星的耐药性. 中华预防医学杂志, 2011, 45（2）:175-177.

［101］Kataria J, Rukmangadachar LA, Hariprasad G, et al. Two dimensional difference gel electrophoresis analysis of cerebrospinal fluid in tuberculous meningitis patients. J Proteomics, 2011, 74(10): 2194-2203.

［102］Kusum S, Aman S, Pallab R, et al. Multiplex PCR for rapid diagnosis of tuberculous meningitis. J Neurol, 2011, 258(10): 1781-1787.

［103］Duo L, Ying B, Song X, et al. Molecular profile of drug resistance in tuberculous meningitis from southwest china. Clin Infect Dis, 2011, 53(11): 1067-1073.

［104］Nagdev KJ, Kashyap RS, Parida MM, et al. Loop-mediated isothermal amplification for rapid and reliable diagnosis of tuberculous meningitis. J Clin Microbiol, 2011, 49(5): 1861-1865.