关键词: 结核, 脑膜, 环氧水解酶类, 多态性, 单核苷酸, 疾病遗传易感性

Abstract: Objective  To investigate the association between the gene polymorphisms of leukotriene A4 hydrolase （LTA4H）and tuberculous meningitis.  Methods  The genotypes and haplotype frequency of LTA4H rs1978331，rs2247570，rs2660898，rs2660845 alleles from 48 patients with tuberculous meningitis and 70 health controls were analyzed by SNaPshot. Differences in allelic and genotypic distributions between the case group and health control group were analyzed by the χ² test, and were considered to be significant when P values were less than 0.05. All data were analyzed using SHEsis software.  Results  The following  frequencies of 3 LTA4H alleles or genotypes between the controls and the cases had all significant differences: rs1978331 alleles between the controls（frequency of C alleles was 59.3% and of T alleles was 40.7%）and the cases(frequency of C alleles was 41.7% and of T alleles was 58.3%) (χ²=7.080, P=0.008), rs1978331 genotypes between the controls(frequency of CC genotype was 17.1%,of CT genotype was 47.1% and of TT genotype was 35.7%) and the cases(frequency of CC genotype was 41.7%,of CT genotype was33.3% and of TT genotype was 25.0%) (χ²=8.670,  P=0.013), rs2660898 genotypes between controls(frequency of AA genotype was 40.0%,of AC genotype was 45.7% and of CC genotype was 14.3%) and cases(frequency of AA genotype was 41.7%,of AC genotype was25.0% and of CC genotype was 33.3%) (χ²=7.980,  P=0.019). The frequency of CACT haplotypes between controls (26.3%) and cases(45.8%) had also a significant difference (χ²=7.349, P=0.007).  Conclusion  One allele(rs1978331),two genotypes(rs1978331,rs2660898) and one haplotype (rs1978331, rs2247570, rs2660898, rs2660845: CACT)in LTA4H gene were correlated with tuberculous meningitis susceptibility in Chinese Han population.

Key words: Tuberculosis, meningeal, Epoxide hydrolases, Polymorphism, single nucleotide, Genetic predisposition to disease