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中国防痨杂志 ›› 2006, Vol. 28 ›› Issue (5): 288-290.

• 论著 • 上一篇    下一篇

结核分枝杆菌接种菌量对异烟肼和利福平药敏试验的影响

黎友伦1；陈保文2；沈小兵2；罗永艾1；王国治2；   

  1. 1重庆医科大学附属第一医院肺科 重庆 400016；2中国药品生物制品检定所 北京 100050；
  • 出版日期:2006-05-10 发布日期:2006-11-03

The influence on Isoniazid and Rifampicin drug susceptibility of the different bacterium quantity in Mycobacterium tuberculosis

Li Youlun,Chen Baowen,Shen Xiaobing,et al.   

  1. 1.Departmet of Pulmonary Disease,The First Affiliated Hospital of Chongqing University of Medical Sciences,Chongqing 400016,China.
  • Online:2006-05-10 Published:2006-11-03

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摘要： 目的比较接种不同菌量结核分枝杆菌（MTB）绝对浓度法药物敏感性（药敏）与比例法药敏的差异程度,为MTB药敏检测准确性提供参考。方法选择临界敏感或临界耐药MTB菌株53株,用比浊法制作MTB菌悬液,10倍梯度稀释,10-4、10-510-6mg/ml菌悬液0.1 ml接种于改良L-J培养基上作活菌计数,10-2、10-3mg/ml菌悬液0.1 ml接种于含INH、RFP改良L-J培养基上作绝对浓度法药敏,10-3、10-5mg/ml菌悬液0.1 ml接种于含INH、RFP改良L-J培养基上作比例法药敏。结果MTB活菌计数值多在5×1065×107CFU/ml。10-2、10-3mg/ml菌悬液绝对浓度法药敏一致率INH、RFP分别达98.1%、100.0%;绝对浓度法与比例法药敏符合率INH、RFP均达90.6%100.0%。结论不同菌量MTB绝对浓度法药敏结果与比例法药敏结果比较一致性好,在常规药敏检测中不会影响结果的准确性。

关键词: 分枝杆菌,结核, 活菌计数, 药敏试验

Abstract: Objective To compare the difference of drug susceptibility of Mycobacterium tuberculosis（MTB） by using both absolute concentration method with different bacterium quantity and proportion method. Methods The 53 probable drug-susceptible or drug-resistant strains of MTB were selected.The bacterial suspensions of MTB were prepared using Mcfarland method.0.1 ml of bacterial suspensions(including 10-4,10-5,10-6 mg/ml) was incubated for MTB CFU,respectively.0.1 ml of bacterial suspensions(including 10-2,10-3 mg/ml) was incubated for the drug susceptibility testing with absolute concentration method,respectively.0.1 ml of bacterial suspensions(including 10-3,10-5 mg/ml) was incubated for the drug susceptibility testing with proportion method,respectively. Results The number of MTB CFU was from 5×106 CFU/ml to 5×107 CFU/ml in Mcfarland method.The accordance rate of isoniazid and rifampicin drug susceptibility in 10-2 and 10-3 mg/ml of MTB was 98.1%,100.0% using absolute concentration method,respectively.The accordance rate of isoniazid and rifampicin drug susceptibility of MTB was from 90.6% to 100.0% with absolute concentration method and proportion method. Conclusion The result of drug susceptibility of MTB using absolute concentration method and proportion method is accordant.

Key words: Mycobacterium tuberculosis, CFU, Drug susceptibility testing

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