肺结核病人T淋巴细胞核仁区酸性非组蛋白表达活性的研究

中国防痨杂志 ›› 2001, Vol. 23 ›› Issue (5): 278-280.

肺结核病人T淋巴细胞核仁区酸性非组蛋白表达活性的研究

袁梅英；孙桂芝；夏东；朱新颖；董建泉；朱慧楠；

北京胸科医院北京 100095；

出版日期:2001-05-10 发布日期:2001-11-03

The expression of nucleolar organizer regions associated of Tlymphocytes in pulmonary tuberculosis

YUAN Mei-ying,SUN Gui-zhi,XIA Dong,et al.

Beijing Chest Hospital,Beijng 100095

Online:2001-05-10 Published:2001-11-03

摘要/Abstract

摘要： 目的　探讨结核病人T淋巴细胞核仁区酸性非组蛋白 (Ag-NORs)的数量，间接反映T淋巴细胞的功能，及其在结核病诊断及发病中的意义。方法使用北京开隆公司提供的KL型免疫图像分析系统对41例正常人，77例肺结核病人和32例肿瘤病人的T淋巴细胞核仁区酸性非组蛋白表达活性进行对比，以核仁染色区面积与细胞核面积之比 (I.S%)定量反映Ag-NORs的多少。结果正常人I.S%为 (7.20±0.95)%，肺结核病人为 (5.59±1.47)%；肿瘤病人为 (5.04±1.52)%，二者与正常人相比有显著性差异 (P＜0.001)。结核病人略高于肿瘤病人，他们之间无显著性差异。结论结核病人酸性非组蛋白表达检测，可用于对结核病人免疫功能的检测，对估计病人的预后有临床指导意义。

关键词: 结核,肺, 核仁区酸性非组蛋白, 免疫,细胞

Abstract: Objective To explore the role in diagnosis and pathogenesis of tuberculosis of nucleolar organizer regions (NORs) associated proteins (Ag-NORs) by which studies the quantity of Ag-NORs was obtained and the function of T lymphocytes was indirectly reflected.Methods 77 cases with pulmonary tuberculosis,32 cases with tumor and 41 controls individuals were analyzed with a KL Imaging System.Results I.S% of patients with tuberculosis (n=77) and with tumor (n=32) was significantly different with that of the normal control (n=41),P＜0.001,while the difference was not found between I.S% of patients with tuberculosis and with tumor (P＞0.05).Conclusion The expression activity assay of Ag-NORs can be used to determine the immune function and evaluate prognosis of patients with pulmonary tuberculosis.

Key words: Tuberculosis,Pulmonary Nucleolar organizer region-associated protein, Immune,cellular

袁梅英,孙桂芝,夏东,朱新颖,董建泉,朱慧楠,. 肺结核病人T淋巴细胞核仁区酸性非组蛋白表达活性的研究[J]. 中国防痨杂志, 2001, 23(5): 278-280.

YUAN Mei-ying,SUN Gui-zhi,XIA Dong,et al.. The expression of nucleolar organizer regions associated of Tlymphocytes in pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2001, 23(5): 278-280.