Influence of malnutrition on positive rate of interferon gamma release assay (IGRA) in patients with tuberculosis

doi:10.19982/j.issn.1000-6621.20210709

Abstract

Abstract:

Objective: To investigate whether malnutrition affects the positive rate of γ-interferon release assay (IGRA) in patients with tuberculosis. Methods: From January 2015 to November 2018, 443 patients with tuberculosis who were admitted to the Department of Respiratory and Critical Care Medicine, West China Hospital were included by reviewing electronic medical record. According to body mass index (BMI), they were divided into malnutrition group (BMI <18.5; 136) and no-malnutrition group (BMI ≥18.5; 307). Enzyme linked immunosorbent assay was used to measure the concentration of interferon-γ in fresh heparinized whole blood stimulated by tuberculosis specific antigen in vitro. The positive rate of IGRA in the two groups was compared. Results: A total of 443 patients, the positive rate of IGRA was 76.47% (104/136) in the malnourished group and 78.18% (240/307) in the no-malnutrition group, there was no significant difference between the two groups (χ²=0.158,P=0.712). Among the bacterium-confirmed tuberculosis patients, the positive rate of IGRA was 75.61% (31/41) in the malnutrition group and 80.65% (50/62) in the no-malnutrition group, there was no significant difference between the two groups (χ²=0.373,P=0.542). Conclusion: Malnutrition does not affect the positive rate of IGRA in patients with tuberculosis.

Key words: Tuberculosis, Interferon-gamma, Immunologic tests, Nutritional assessment, Comparative study

CLC Number:

LA Ba, ZHANG Meng-meng, WANG Ming-gui, HE Jian-qing. Influence of malnutrition on positive rate of interferon gamma release assay (IGRA) in patients with tuberculosis[J]. Chinese Journal of Antituberculosis, 2022, 44(6): 595-599. doi: 10.19982/j.issn.1000-6621.20210709

Figures/Tables 4

References 23

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一般特征	营养不良组(136例)	非营养不良组(307例)	统计检验值	P值
年龄[岁,M(Q₁,Q₃)]	29.0(20.0,49.0)	39.0(27.0,52.0)	Z=-3.398	0.001
性别[例(构成比,%)]			χ²=7.239	0.007
男性	90(66.18)	161(52.44)
女性	46(33.82)	146(47.56)
身高[cm,M(Q₁,Q₃)]	165.0(160.0,170.8)	163.0(158.0,170.0)	Z=2.782	0.006
体质量[kg,M(Q₁,Q₃)]	47.0(42.0,50.0)	58.0(51.0,64.0)	Z=-16.903	<0.001
吸烟[例(发生率,%)]	53(38.97)	91(29.64)	χ²=3.739	0.053
饮酒[例(发生率,%)]	36(26.47)	68(22.15)	χ²=0.979	0.322
病原学检测任一阳性[例(阳性率,%)]	41(30.15)	62(20.20)	χ²=5.231	0.022

检测结果	营养不良组 (136例)	非营养不良组 (307例)	χ²值	P值
所有患者			0.158	0.712
阳性	104(76.47)	240(78.18)
阴性	32(23.53)	67(21.82)
病原学阳性患者			0.373	0.542
阳性	31(75.61)	50(80.65)
阴性	10(24.39)	12(19.35)

T淋巴细胞亚群	营养不良组(136例)	非营养不良组(307例)	Z值	P值
淋巴细胞计数[×10⁹/L,M(Q₁,Q₃)]	1.1(0.7,1.4)	1.2(0.8,1.6)	-1.866	0.062
CD3亚群[%,M(Q₁,Q₃)]^a	73.3(65.7,80.2)	73.0(66.1,79.5)	-0.223	0.824
CD4亚群[%,M(Q₁,Q₃)]^a	38.8(33.0,45.8)	38.3(29.6,45.7)	-0.474	0.635
CD8亚群[%,M(Q₁,Q₃)]^a	27.2(20.8,31.9)	26.2(20.0,33.0)	-0.609	0.542

淋巴细胞	IGRA阳性(89例)	IGRA阴性(29例)	统计检验值	P值
淋巴细胞计数[×10⁹/L,M(Q₁,Q₃)]	1.1(0.7,1.4)	0.9(0.6,1.4)	Z=-0.581	0.561
淋巴细胞[例(构成比,%)]			χ²=1.399	0.237
正常或增加	45(50.56)	11(37.93)
减少	44(49.44)	18(62.07)
CD3亚群[%,M(Q₁,Q₃)]	73.6(67.4,80.9)	66.4(63.9,78.3)	Z=-1.141	0.254
CD4亚群[%,M(Q₁,Q₃)]	39.2(32.7,45.4)	38.4(33.1,49.0)	Z=-0.056	0.955
CD8亚群[%,M(Q₁,Q₃)]	27.5(20.8,34.5)	24.6(20.5,29.3)	Z=-1.664	0.096