Abstract: Objective To study the application value of testing TB-SA genes of Mycobacterium tuberculosis in tuberculosis diagnosis. Methods Of 371 tuberculosis cases, 307 cases with pulmonary tuberculosis and 64 cases with nonpulmonary tuberculosis were selected. Sixty-one cases suffered from non-tuberculosis respiratory diseases such as pneumonia or lung cancer. Forty cases were selected as healthy control. The TB-SA gene was amplified by PCR, bacteriologic (smear and culture) using same sputum,thoracic,abdominal or cerebrospinal fluid. Result 259 of 307 cases PCR positive, sensation is 84.4% (259/307), and that the bacteriologic sensation is 62.5%(192/307). Among the 64 extra-pulmonary patients, TB-SA gene positive 35 cases, sensation is 54.7% (35/64); and the bacteriologic sensation is 7.8%(5/64). Meanwhile the result of 61 cases non-tuberculosis respiratory patients and the 40 healthiness, the especial are 96.7%(59/61) and 100%(40/40). Conclusion The especial and sensation of this kit was content. operating easily and could be apply popularly.

Key words: Mycobacterium tuberculosis, PCR, TB-SA genes, tuberculosis/diagnosis