First isolation, identification and characterization of Mycobacteriophages in Yunnan Province

doi:10.19982/j.issn.1000-6621.20250248

Abstract

Abstract:

Objective: To isolate Mycobacteriophages from Yunnan Province and elucidate their phenotypic and genomic characteristics. Methods: Hospital sewage samples, soil samples, and fecal samples were collected in Kunming, Dali, Nujiang, Dehong, Yuxi, Lincang, Pu'er, Zhaotong, and Baoshan cities in Yunnan Province. Using Mycobacterium smegmatis mc²155 as the host bacterium, direct and enrichment methods were used to isolate Mycobacteriophages from the sample. The isolated Mycobacteriophages were subjected to biological characterization, whole genome sequencing, and genome analysis. Results: Three Mycobacteriophages were isolated from 186 samples, and one strain named YN1 was selected for the further study. The results showed that the optimal multiple number of YN1 infection was 0.1, and the growth incubation period was about 330 minutes. Environmental factors such as temperature, pH, UV and ethanol had certain effects on YN1.Genomic analysis showed that YN1 belongs to the linear double stranded DNA virus, while comparative genomics indicated that YN1 belongs to the mycobacterial Ⅰ cluster bacteriophage in taxonomy. Conclusion: This study isolated Mycobacteriophages from Yunnan Province for the first time. After identification and characterization, YN1 was identified as a new mycobacterial cluster Ⅰ bacteriophage, enriching the domestic mycobacterial bacteriophage library and providing a basis for further development and utilization of Mycobacteriophages.

Key words: Mycobacteriophages, Biology, Genome, Phenotype, Yunnan Province

CLC Number:

R378.91

Du Yu, Zhang Haipeng, Liu Kejun, Shi Liyuan, Tan Hongli, Wang Peng. First isolation, identification and characterization of Mycobacteriophages in Yunnan Province[J]. Chinese Journal of Antituberculosis, 2025, 47(12): 1609-1615. doi: 10.19982/j.issn.1000-6621.20250248

Figures/Tables 5

References 33

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感染复数	样本数(份)	效价[PFU/ml,M(Q₁, Q₃)]
0.001	3	6.00×10³(4.00×10³,10.0×10³)
0.01	3	6.00×10⁵(4.00×10⁵,8.00×10⁵)
0.1	3	1.20×10⁷(1.00×10⁷,1.40×10⁷)
1	3	4.00×10⁶(2.00×10⁶,4.00×10⁶)
10	3	1.60×10⁶(1.00×10⁶,1.80×10⁶)
100	3	2.40×10⁵(1.60×10⁵,2.40×10⁵)
1000	3	6.00×10⁴(4.00×10⁴,8.00×10⁴)

乙醇浓度(%)	样本数(份)	效价[PFU/ml,M(Q₁, Q₃)]	H值	P值
0	3	1.60×10⁷(1.50×10⁷,1.60×10⁷)	25.800	<0.001
10	3	8.00×10⁶(8.00×10⁶,10.00×10⁶)
25	3	8.00×10⁵(6.00×10⁵,8.00×10⁵)
50	3	0.00
75	3	0.00
95	3	0.00