Comparison of phage-amplified biologically assay and conventional proportion method for the drug susceptibility testing in Mycobacterium tuberculosis

Abstract

Abstract: Objective To evaluate the value of phage-amplified biologically assay (PhaB),to set up a fast,sensitive,specific and inexpensive method for testing the drug susceptibility in Mycobacterium tuberculosis.Methods Fifty-two M.tuberculosis isolates were tested the susceptibility to rifampicin and isoniazid by using the PhaB assay and the conventional proportion method.Results Using the conventional proportion method as control,the sensitivity of testing using the PhaB assay were 94.1% (32/34) on rifampicin-resistant isolates,and 92.1% (35/38) on isoniazid-resistant isolates,the specificity were 94.4% (17/18) on rifampicin-sensitive isolates,and 78.6% (11/14) on isoniazid-sensitive isolates.The two susceptibility Methods for rifampicin and isoniazid were 94.2% and 88.5% agreement respectively.Conclusion The PhaB assay could test correctly the drug susceptibility on the majority of M.tuberculosis isolates in 2 to 4 days.It might become a drug susceptibility testing with simplicity,high sensitivity,high specificity and low cost.

Key words: Bacteriophage, Rifampicin, Isoniazid, Mycobacterium tuberculosis, Drug susceptibility test

Peng Li,Chen Baowen,Shen Xiabing,et al.. Comparison of phage-amplified biologically assay and conventional proportion method for the drug susceptibility testing in Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2005, 27(1): 18-22.

References

1 WHO/IUATLD Global Project on Anti-Tuberculosis Drug Resistance Surveillance, 1997. Anti-tuberculosis drug resistance in the world [R]. World Health Organization publication, WHO/TB/97,227.
2 Wilson S M , Al Suwaidi Z, McNerny K,et al. Evaluation of a new rapid bacteriophage-based method for the drug susceptibility testing of Mycobacterium tuberculosis [J]. Nat Med，1997,3(4):465～468.
3 Jacobs W R, Barletta R, Udani R,et al. Comparison of drug susceptibility of Mycobacterium tuberculosis by means of luciferase reporter phage[J].Science,1993,260:819-822.
4 Albert H，Heydenrych A，Brookes H, et al. Performance of a rapid phage-based test, FAST Plaque TB^TM, to diagnose pulmonary tuberculosis from sputum specimences in South Africa [J]. Int J Tuberc: Lung Dis，2002,6(6):529～539.
5 Marie A M , EI-Behedy E M, Mohtady H A，et al. Evaluation of a rapid bacteriophage-based method for the detection of Mycobacterium tuberculosis in clinical samples [J]. J Med Microbiol,2003，52(4):331～335.
6 Eltringham I J, Wilson S M, Drobnieski F A. Evaluation of a bacteriophage-based assay (phage amplified biologically assay) as a rapid screen for rapid screen for resistance to isoniazid, ethambutol, strep-toniycin, pyrazinamide and ciprofloxacin among clinical isolates of Mycobacterium tuberculosis [J]. J Clin Microbiol, 1999,37 (11)：3528～3532.
7 McNerny R, Kiepiela K S,Bishop P M, et al. Rapid screening of Mycobacterium tuberculosis for susceptibility to rifampin and strep to mycine [J].Int J Tuberc Lung Dis, 2000.4：1～7.
8 McNerny R, Wilson S M, Sidhu A M，et al. Inactivation of mycobacteriophage D29 using ferrous ammonium sulphate as a tool for the detection of viable mycobacterium smegmatis and mycobacterium tuberculosis [J]. Res Microbiol, 1998,149:487～495.
9 Watterson S A, Wilson S M, Yates M D,et al. Comparison of three molecular assays for detection of rifampin resistance in Mycobacterium tuberculosis [J]. J Clin Microbiol, 1998，36(7)：1969～1973.
10 胡忠义,庞银,靳安佳等.应用噬菌体裂解法快速鉴定结核分枝杆菌[J].中华结核和呼吸杂志,2001,24(10):611～613.
11 Sambrook J，Fritsch E F，Maniatis T. Molecular Cloning： A laboratory Manual 2nd edition [M]. Cold Spring Harbor Laboratory　press，1998.

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