Abstract: Objective To set up a method of Rapid Fluorimetry for identification of group of Mycobacterium by using the liquid culture medium.Methods Examined the drug susceptibility in vitro of 15 strains standard Mycobacterium and 78 strains Mycobacterium from clinical samples to P Nitro benzoic Acid (PNB) and 2 Thiophene carborylic acid hydrazide (TCH) by the examining tube of radioactive Free and rapid fluorimetry anddetermined the concentration of PNB and TCH in testing of identification of group of Mycobacterium.Results 100μg/ml of PNB can identify Mycobacterium tuberculosis complex and non tuberculosis mycobacterium (NTM),2.5μg/ml TCH can identify mycobacterium tuberculosis and Mycobacterium bovis .The test just took 7～10 days.Conclusion This method is economic,practical and the report time was shorten significantly for identification of group of mycobacterium.The report time of the method was 7～10 days.It was 18～21 days shorter than that of the method of L J (28 days).

Key words: Mycobacterium, Identification of group of mycobacterium, Fluorometry tube, P-Nitro benzoic Acid (PNB), 2-Thiophene carborylic acid hydrazide (TCH)