Abstract: Objective To probe possible role of HLA-DQB1-HLA-DRB1 haplotype in incidence of pulmonary tuberculosis among Han nationality of southern Chinese.Methods PCR-SSP was applied to detect 20 alleles of HLA-DRB1 and 8 alleles of HLA-DQB1 gene sites of 110 tuberculosis cases and 101 controls who came from Guangdong,Guangxi,Hunan,Hubei,Jiangxi and Fujian in a case control study.Then DQ2,3(8)-DRB1,DQ3(7)-DRB1,DQ3(8,9)-DRB1,DQ2,3(7,9)-DRB1,DQ2-DRB1,DQ4-DRB1,DQ5-DRB1 and DQ6-DRB1 haplotype frequencies (HF) as well as relative risks (RR) were calculated and compared.Results It was found that frequencies of DQ2,3(8)-DR12.1,DQ3(7)-DR16 haplotype in pulmonary tuberculosis cases were strikingly higher than that in healthy controls (6.10 vs.0.50、4.18 vs.0.99,their RRs were 13.40 and 4.41) and that frequencies of DQ2-DR1,DQ2-DR12,DQ2-DR13.3,DQ3(7)-DR1,DQ3(7)-DR13.3,DQ3(8,9)-DR13.3,DQ2,3(7,9)-DR1,DQ2,3(7,9)-DR13.3,DQ2,3(7,9)-DR13.4,DQ4-DR4 haplotype in pulmonary tuberculosis cases were remarkable lower than that in healthy controls (1.84 vs.5.60,1.37 vs.5.60,4.18 vs.11.00,2.30 vs.9.89,12,62 vs.22.28,5.61 vs.11.56,3.70 vs.14.40,16.88 vs.28.94,5.13 vs.12.12,2.30 vs.6.13),which their RRs were 0.31,0.23,0.34,0.21,0.47,0.44,0.46,0.38 and 0.35 respectively.Conclusion This study suggested that DQ2,3(8)-DR14.1,DQ2-DR12,DQ2,3(7,9)-DR13.4,DQ4-DR4 haplotype is potential relative to incidence of pulmonary tuberculosis of Han nationality of southern Chinese.However,differences of frequencies of DQ3(7)-DR16,DQ2-DR1,DQ2-DR13.3,DQ3(7)-DR1,DQ3(7)-DR13.3,DQ3(8,9)-DR13.3,DQ2,3(7,9)-DR1,DQ2,3(7,9)-DR13.3 haplotype between pulmonary tuberculosis cases and healthy controls may be caused by remarkable differences of gene frequency (GF) of constituted gene.

Key words: Pulmonary tuberculosis, HLA, Haplotype, PCR-SSP