Research progress on nanopore-based targeted sequencing in identification of Mycobacterium tuberculosis and detection of drug-resistant genes

doi:10.19982/j.issn.1000-6621.20240049

Abstract

Abstract:

The Global Tuberculosis Report 2023 showed that the epidemiological situation of tuberculosis is still serious. The current detection methods exhibit limitations in accurately identifying mycobacterial species and detecting drug-resistant genes. There is an urgent need to develop clinically applicable detection technologies that are more convenient, efficient, and precise to adapt the needs for early diagnosis and treatment of tuberculosis. Nanopore sequencing presents a promising avenue with its inherent advantages of convenience, efficiency, long-read lengths, and extensive sequencing capabilities, thereby offering novel prospects for diagnosing and treating tuberculosis as well as drug-resistant strains. This paper provides a comprehensive review of the principles and characteristics of nanopore-based targeted sequencing along with its applications in mycobacterial identification and drug-resistant gene detection, aiming to provide references for clinical staff to explore the innovative technology.

Key words: Mycobacterium tuberculosis, Genes, Diagnosis

CLC Number:

R52
R446.5

Yang Chen, Gao Weiwei, Guo Yicheng, Zeng Yi. Research progress on nanopore-based targeted sequencing in identification of Mycobacterium tuberculosis and detection of drug-resistant genes[J]. Chinese Journal of Antituberculosis, 2024, 46(5): 578-583. doi: 10.19982/j.issn.1000-6621.20240049

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