重组结核分支杆菌38kD和16kD蛋白用于结核病血清学诊断的价值

中国防痨杂志 ›› 2001, Vol. 23 ›› Issue (5): 281-283.

重组结核分支杆菌38kD和16kD蛋白用于结核病血清学诊断的价值

张小刚¹；庄玉辉¹；何秀云¹；熊志红¹；董恩军¹；李书琳²；张永胜²；李昕²；

1.解放军第三○九医院结核病研究室北京 100091；2.吉林省吉林市结核病防治研究所吉林 132011；

出版日期:2001-05-10 发布日期:2001-11-03

Evaluation of the recombinant 38kD and 16kD antigen of Mycobacterium Tuberculosis for serodiagnostic test

ZHANG Xiao-gang,ZHUANG Yu-hui,HE Xiu-yun,et al.

Tuberculosis Research Lab,The 309th hospital of PLA.Beijing 100091

Online:2001-05-10 Published:2001-11-03

摘要/Abstract

摘要： 目的　探讨国产重组结核分支杆菌蛋白38kD(rTPA38)和16kD(rTPA16)用于结核病的诊断价值。方法以rTPA38和rTPA16为抗原，PPD为对照，用ELISA法检测血清中特异性抗结核抗体。结果 246例肺结核病组，rTPA38、rTPA16和PPD检测的灵敏度分别为66.3%、63.0%和72.3%。健康献血组、非结核呼吸疾病组和卡介苗接种阳转组血清同时用rTPA38、rTPA16和PPD检测，rTPA38特异性分别为97.6%、96.8%、86.0%。rTPA16特异性分别为94.7%、93.1%、75.0%。PPD特异性为93.4%、85.7%、67.9%。统计分析显示，rTPA38蛋白和PPD检测非结核呼吸疾病组，其阳性率有显著性差异 (P＜0.05)。两者检测卡介苗接种阳转组阳性率和血清抗体滴度有显著性差异 (P＜0.05)。rTPA38和rTPA16同时检测108例肺结核病组血清可提高11.1%的阳性率。结论 rTPA38蛋白抗原有较好的灵敏度和较高特异性，是ELISA的可靠抗原，与rTPA16联用可提高灵敏度，对结核病血清学诊断有较高参考价值。

关键词: 分支杆菌,结核, 重组蛋白38kD、16kD, ELISA血清学诊断

Abstract: Objective The recombinant 38kD and 16kD protein of Mycobacterium tuberculosis (rTPA38 and rTPA16) as serological diagnostic reagents were evaluated.Methods The purified rTPA38 and rTPA16 were used to detect sera antibodies using enzyme-linked immunosorbent assay (ELISA),and using the PPD as control.Results In detecting the sera of 246 pulmonary tuberculosis patients,the sensitivity of rTPA38,rTPA16 and PPD was 66.3%,63.0% and 72.3% respectively.The specificity of rTPA38,rTPA16 and PPD in detecting of other groups:healthy controls,non tuberculosis respiratory patients,BCG-vaccinated healthy controls,was 97.6%, 96.8%, 86.0%;94.7%,93.1%,75.0% and 93.4%,85.7%,67.9% respectively.In detecting the sera of non tuberculosis respiratory patients and BCG vaccinated healthy controls,rTPA38 had much more positive reactions than that of PPD.To the sera of pulmonary tuberculosis patients,using combination of rTPA38 and rTPA16 as detecting antigens can improve the positive rate by 11.1%.Conclusion rTPA38,which can provide a satisfactory sensitivity and specificity,may become effective antigen in ELISA.A combination of rTPA38 and rTPA16 can improve the sensitivity.

Key words: Mycobacterium tuberculosis, 38kD Protein, 16kD Protein, ELISA, Serological diagnosis

张小刚,庄玉辉,何秀云,熊志红,董恩军,李书琳,张永胜,李昕,. 重组结核分支杆菌38kD和16kD蛋白用于结核病血清学诊断的价值[J]. 中国防痨杂志, 2001, 23(5): 281-283.

ZHANG Xiao-gang,ZHUANG Yu-hui,HE Xiu-yun,et al.. Evaluation of the recombinant 38kD and 16kD antigen of Mycobacterium Tuberculosis for serodiagnostic test[J]. Chinese Journal of Antituberculosis, 2001, 23(5): 281-283.