关键词: 甘露聚糖结合凝集素, 结核/治疗, 免疫疗法

Abstract: Objective To study the immunotherapeutic effects of MBL(mannan-binding lectin) on murine infected with Mycobacterium tuberculosis.Methods Eighteen BALB/ c mice were randomized into 3 groups,One group treated with mannan-binding lectin,another group treated with vaccae vaccine,The third group treated with PBS(n=6).1×10⁶ bacili in a volume of 300 μL PBS were injected into the tail vein of mice in each group.The mice from each group were treated intraperitoneally with MBL,Vaccae(freeze-dried M.vaccae vaccine for therapy) and PBS at 2 weeks postinfection,respectively.The animals were sacrificed at 2 weeks after treatment.The weights of mice,lungs and spleens were measured.Then the lungs and spleens of mice were processed for histological examination.The numbers of viable bacteria in lungs and spleens were counted.Results At 2 weeks after therapy,the body weights of mice in treatment groups were higher than that in control group,the lung weights of mice in treatment groups were lower than that in control group,the spleen weights of mice in treatment groups were higher than that in control group.The numbers of viable bacteria in lungs and spleens of mice from treatment groups were lower than that in control group,respectively.MBL could improve host defense against Mycobacterium tuberculosis and reduce mycobacteria outgrowth on murine with tuberculosis.Conclusion MBL had immunotherapeutic effects on murine with tuberculosis.

Key words: Mannan-binding lectin, Tuberculosis/therapy, Immunotherapy