Chinese Journal of Antituberculosis ›› 2023, Vol. 45 ›› Issue (6): 566-574.doi: 10.19982/j.issn.1000-6621.20230059
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He Ping1, Wang Yiting1, Song Zexuan1, Xia Hui2, Wang Shengfen2, He Wencong1, Zheng Yang2, Zhao Yanlin2()
Received:
2023-03-01
Online:
2023-06-10
Published:
2023-06-06
Contact:
Zhao Yanlin
E-mail:zhaoyl@chinacdc.cn
Supported by:
CLC Number:
He Ping, Wang Yiting, Song Zexuan, Xia Hui, Wang Shengfen, He Wencong, Zheng Yang, Zhao Yanlin. Preliminary study on the gene function of Mycobacterium tuberculosis Rv2333c in macrophages[J]. Chinese Journal of Antituberculosis, 2023, 45(6): 566-574. doi: 10.19982/j.issn.1000-6621.20230059
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细胞因子 时间(h) | 2-ΔΔCt值 | t值 | P值 | |
---|---|---|---|---|
Ms_Vec组 | Ms_Rv2333c组 | |||
IL-1β | ||||
6 | 657.43±20.96 | 521.36±25.97 | 5.767 | 0.005 |
24 | 292.69±3.23 | 454.56±24.57 | 9.237 | <0.001 |
48 | 1548.53±125.17 | 2168.13±130.00 | 4.855 | 0.008 |
IL-6 | ||||
6 | 2.46±0.49 | 6.02±0.37 | 8.234 | 0.001 |
24 | 490.14±55.19 | 4546.44±186.66 | 29.470 | <0.001 |
48 | 776.41±15.38 | 1642.76±51.15 | 22.940 | <0.001 |
IFN-γ | ||||
6 | 2.52±0.09 | 17.24±0.47 | 43.760 | <0.001 |
24 | 1.55±0.20 | 5.56±0.15 | 22.580 | <0.001 |
48 | 2.76±0.32 | 8.52±0.08 | 25.090 | <0.001 |
蛋白 时间(h) | 灰度值(%, 与GAPDH表达量相比较) | t值 | P值 | |
---|---|---|---|---|
Ms_Vec | Ms_Rv2333c | |||
NF-κB | ||||
3 | 1.00±0.14 | 1.85±0.29 | 3.765 | 0.020 |
6 | 1.00±0.20 | 1.82±0.19 | 4.176 | 0.014 |
12 | 1.00±0.11 | 1.37±0.11 | 3.442 | 0.026 |
24 | 1.00±0.14 | 1.45±0.15 | 3.041 | 0.038 |
p-ERK1/2 | ||||
3 | 1.00±0.06 | 1.74±0.03 | 15.040 | <0.001 |
6 | 1.00±0.03 | 1.36±0.03 | 11.740 | <0.001 |
12 | 1.00±0.05 | 1.22±0.07 | 3.857 | 0.018 |
24 | 1.00±0.02 | 1.17±0.04 | 5.538 | 0.005 |
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