Abstract:

Objective To explore the difference of plasma metabolomics between diabetes (DM) patients and diabetes complicated with tuberculosis (DM-PTB). Methods DM patients (DM group, n=10) and DM-PTB patients (DM-PTB group, n=10) diagnosed in Heilongjiang Provincial Infectious Disease Prevention and Treatment Hospital from January to June 2017 were collected. Venous blood were collected from these patients (10 ml per patient) with empty stomach in the early morning using Ethylenediaminetetraacetic acid (EDTA) anticoagulation tubes, and then the supernatant was collected by centrifugation. Metabolomics detection on the plasma samples of the two groups was performed by UPLC-Q-TOF MS technology, and then multivariate statistical analysis methods were used to compare their metabolic profiles and screen potential different metabolites. Results The plasma metabolism profiles of DM group and DM-PTB group were significantly different; 7 metabolites were preliminarily identified different between the two groups. Of them, the plasma 6-keto-prostaglandin F1a (multiple of difference 143.34, P=0.020), protoporphyrinogen Ⅸ (multiple of difference 33.18, P=0.040), linoleic acid (multiple of difference 8.18, P=0.000), 5-hydroxyeicosatetraenoic acid (multiple of difference 15.09, P=0.000), prostaglandin E2 (multiple of difference 8.88, P=0.000) and leukotriene B4 (multiple of difference 39.58, P=0.000) of the DM-PTB group were higher than those of the DM group, while hippuric acid content of the DM-PTB group (multiple of difference 2.69, P=0.010) was lower than that of the DM group. Conclusion Metabolism of arachidonic acid, linoleic acid, and amino acid happen changes after DM patients complicated with PTB, the above-mentioned plasma metabolites may be early screening indicators for DM-PTB.

Key words: Diabetes mellitus, Pulmonary tuberculosis, Comorbidity, Metabolomics, Plasma, Comparative study