Abstract:

Objective To evaluate the application value of three tuberculosis (TB) antibody kits in tuberculosis diagnosis.Methods Of 601 patients treated by PCR-reverse dot blot hybridization (PCR-RDB) to identify the tissue species mycobacteria strains (referred to as molecular pathology) at Department of Pathology, the 309th Hospital of PLA between January 2015 and December 2015, the patients who did not meet inclusion criteria were excluded. Finally, 205 cases were included, and the results of peripheral blood tuberculosis antibody by three TB antibody kits were detected from records. The McNemar test and Kappa test results were used to decide the difference and consistency by SPSS 20.0.Results Compared with molecular pathological results, the sensitivities of TB-IgG, SD Rapid TB, TB-DOT were 26.6% (34/128), 20.3% (26/128), 97.7% (125/128); the specificities were 90.9% (70/77), 98.7% (76/77), 3.9% (3/77); the positive predictive values were 82.9% (34/41), 96.3% (26/27), 62.8% (125/199); the negative predictive values were 42.7% (70/164), 42.7% (76/178), 50.0% (3/6); the jaden indexes were 0.18, 0.19, 0.02, respectively. Compared with the molecular pathology group, diagnosis differences in TB-IgG and SD Rapid TB kits groups were statistically significant (χ ²=9.17, P<0.001; {\chi }_{\mathrm{Correct}}^2 =13.58, P<0.001). TB-IgG and SD Rapid TB kits were found weak consistency with the molecular pathology (Kappa=0.14, 0.15, Ps<0.001);while diagnosis difference between the molecular pathology and TB-DOT kit was not statistically significant (χ ²=0.04, P=0.833), and consistency was not sure (Kappa=0.02, P=0.523). Conclusion The detection results of three TB antibody kits were different to some extent; SD Rapid TB was relatively better than the other two; multiple antigen detection could improve sensitivity and specificity, and might be assist to the diagnosis of tuberculosis.

Key words: Tuberculosis, Pathology, molecular, Antigen-antibody reactions, Laboratory techniques and procedures, Diagnosis, Evaluation studies