Application of LAMP method in rapid detection of Mycobacterium tuberculosis

Abstract

Abstract: Objective To develop a loop-mediated isothermal amplification (LAMP) method for the detection ofMycobacterium tuberculosis .  Methods 11 standard strains were used to develop the LAMP method in TB detection. The sensitivity and specificity of LAMP were evaluated using 47M. tuberculosisand nontuberculosis mycobacteria isolates and 13 food borne strains.  Results The detection limit of LAMP was 1pg of DNA, the specificity was 97.4%.  Conclusion s The LAMPis a rapid, sensitive, and specific method for the detection ofMycobacterium tuberculosis .

Key words: Mycobacterium tuberculosis, nucleic acid amplification techniques

CLC Number:

R446.5

LIN Shi-Ping, YANG Ying-Zhou, TAN Wei-Guo, LIN Yi-Man, WANG Hong-Fu. Application of LAMP method in rapid detection of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2010, 32(8): 52-55.

References

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