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Chinese Journal of Antituberculosis ›› 2012, Vol. 34 ›› Issue (8): 527-531.

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The practical evaluation of second-line anti-tuberculosis drug susceptibility test using MGIT 960 system

JIANG Jun, ZHANG Juan,ZHANG Hong, JIA Lin, WEI Cheng-cui, SHI Lian, MA Jing-hong, YANG Li-jun   

  1. TB laboratory of Shenyang Chest hospital, Shenyang 110044,China
  • Received:2012-01-30 Online:2012-08-10 Published:2012-08-10
  • Contact: JIANG Jun E-mail:xkyyjykjj@sina.com

Abstract: Objective  To evaluate the practicality of automatic BACTEC MGIT960 system in second-line anti-tuberculosis (TB) drug susceptibility test.  Methods 212 M. tuberculosis strains were isolated and idenfied from the sputum specimen in 435 cases by the smear, Lowenstein-Jenson (L-J) solid culture and MGIT960 liquid culture and identification. These strains were determined the resistance to first-line anti-TB drugs including isoniazid (INH), rifampicin (RFP),streptomycin (S) and ethambutol (EMB) by L-J proportion method, respectively. Then the first-line anti-TB drug-resistant strains were further determined the resistance to second-line anti-TB drugs including capreomycin (Cm), kanamycin (Km),ofloxacin (Ofx) and ethionamide (Eto)  by L-J and MGIT960, respectively.  The detection time and accordance rate of the two methods were compared.  Results The times of drug susceptibility test by MGIT960 and L-J method were average 10.0 days (1183/118) and 28.5 days (3360/118), respectively. There was difference of 18.5 days (28.5-10.0) between two methods. The accordance rates of MGIT960 and L-J method in Cm, Km, Ofx and Eto susceptibility test were 88.1%(104/118), 95.8%(113/118), 91.5%(108/118) and 71.2%(84/118).   Conclusion The second-line anti-TB drug susceptibility test using MGIT960 system is fast, accurate and has higher practical value.

Key words: Mycobacterium tuberculosis, Antibiotics,antitubercular, Microbial sensitivity tests, Bacteriological techniques, Culture media