Polymorphism Analysis of gene iniB that encodes the hypothetical protein Rv0341 of M. tuberculosis by RFLP

Chinese Journal of Antituberculosis ›› 2010, Vol. 32 ›› Issue (11): 756-759.

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Polymorphism Analysis of gene iniB that encodes the hypothetical protein Rv0341 of M. tuberculosis by RFLP

Wu Yuan^1,2; Hu Pinpin³; Wang Yiwei³; Li Xiuquan¹; Mao Xuhu²; Zhong Min^3,4

1.254 PLA Hospital; Tianjin; 300142; China; 2.Department of Clinical Microbiology and immunolgy; college of laboratory medicine; Third Military Medical University-Chongqing Biopharmacenticals Engineering Technology Research Center; Chongqing 400038; 3.Central Laboratory of Genetic Diagnosis of Tuberculosis of Chongqing; Chongqing 400020; 4.Chongqing Infectious Disease Medical Center; Chongqing 400036; China;

Online:2010-11-15 Published:2011-08-23

Abstract

Abstract: Objective To investigate the polymorphism and conservatism of iniB gene that encodes the hypothetical protein Rv0341 of M.tuberculosis,find the possible reason that Rv0341 highly expressed when M.tuberculosis was cultured in the medium containing rifampin.Methods E.coli was used as negative control,the iniB genes from 14 rifampin-dependent M.tuberculosis isolates,23 rifampin-resistant isolates,12 rifampin-sensitive isolates,M.tuberculosis H37Rv and BCG were amplified by PCR,and then digested by restriction ...

Key words: M.tuberculosis, bacterial proteins, polymorphism, restriction fragment length

WU Yuan, HU Pin-Pin, WANG Yi-Wei, LI Xiu-Quan, MAO Xu-Hu, ZHONG Min. Polymorphism Analysis of gene iniB that encodes the hypothetical protein Rv0341 of M. tuberculosis by RFLP[J]. Chinese Journal of Antituberculosis, 2010, 32(11): 756-759.

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Polymorphism Analysis of gene iniB that encodes the hypothetical protein Rv0341 of M. tuberculosis by RFLP

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