Study on the relationship between polymorphism of BTNL2 gene and susceptibility of tuberculosis in Chinese population

Abstract

Abstract: ObjectiveTo investigate the genetic polymorphisms of BTNL2 gene in Chinese, and to study the possible correlation of BTNL2 polymorphism and the susceptibility to tuberculosis. MethodsBlood samples from 94 tuberculosis cases and 95 controls were collected for a case-control study in Chinese. DNAs of the subjects were extracted and amplified by polymerase chain reaction (PCR). The single nucleotide polymorphisms (SNPs) of BTNL2 were determined by SNaPshot SNP genotype techniques. The genotype and haplotype frequencies were compared between tuberculosis cases and controls using SHEsis software. ResultNo correlation was detected between any of the single SNPs locus studied and disease. However, the C-A-T-G-C-G and C-G-T-G-C-G haplotype groups were present a significant correlation with susceptibility to tuberculosis, with the OR (95% CI) to be 0.39 (0.19～0.79) and 3.50 (0.93～13.18) respectively. ConclusionThe single SNP in polymorphisms of BTNL2 gene might not be correlated with the susceptibility to tuberculosis in Chinese. But, the haplotype of BTNL2 gene may be correlated with tuberculosis.

Key words: tuberculosis,pulmonary/genetics, disease susceptibility, membrane glycoproteins, polymorphism,single nucleotide

Lian Yuanyuan, Yue Jun, Han Min, Liu Jianfang, Liu Lirong. Study on the relationship between polymorphism of BTNL2 gene and susceptibility of tuberculosis in Chinese population[J]. Chinese Journal of Antituberculosis, 2009, 31(4): 199-205.

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