Abstract: Objective To evaluate the diagnostic value of tuberculin protein polypeptide (TPP) and microwaves irradiation in detective of active tuberculosis.Methods Tuberculin protein polypeptide was drawed from culture medum of M.Tuberculosis H₃₇ Rv,was used microwaves-enzyme linked immunosorbent assay,to determine the titer of serum antibody against TPP in active tuberculosis.Results It was found that the tests were positive(≥1：80)in 114 of 126 active tuberculosis cases and in 10 of 76 healthy individuals.The sensitivity was 90.4% and the specificity was 86.8%.Besides,compared with the conventional-enzyme linked immunosorbent assay,the consumptive time of microwaves-enzyme linked immunosorbent assay was reduced considerably,but gain a consistent conclusion.Conclusion The antigen of TPP was proved to be better in sensitivity and specificity.Microwaves-enzyme linked immunosorbent assay can substitute conventional-enzyme linked immunosorbent assay.

Key words: Tuberculin protein polypeptide, Microwaves-enzyme linked immunosorbent assay, Tuberculosis,pulmonary