Abstract: Objective To evaluate the diagnostic value for renal tuberculosis by detection of Mycobacterium tuberculosis from Urine with phage amplified biologically(phaB) assay. Methods 63 patients with renal tuberculosis and 21 healthy subjects were recruited as research group and control, respectively. We detected Mycobacterium tuberculosis in the urine from research group patient using smear, culture and phaB. Meanwhile TB-DNA were detected from the urine by polymerase chain reaction(PCR). The urine from the control were detected using phaB and PCR.  Results The positive rate of detection for Mycobacterium tuberculosis from urine by phaB in research group was significant higher than that by smear and culture (P<0.01). But the positive rate was no significant difference between using phaB and using PCR (P>0.05), Specificity and sensitivity of phaB for detecting Mycobacterium tuberculosis from urine was 95.2% and 69.8%, respectively.  Conclusion PhaB is useful tool to detectMycobacterium tuberculosisfrom urine for diagnosis of renal tuberculosis.

Key words: tuberculosis,renal/urine, Mycobacterium tuberculosis, bacteriophages