Abstract: Objective  To explore the factors affecting job satisfaction of health care workers in county-level TB institutions of Hunan province.   Methods  660 health care workers (HCWs) were sampled by stratified random sampling method from 110 county level TB institutions of Hunan province, and they were given the job satisfaction questionnaire survey. The content of the survey include seven dimensions: leadership behavior, management system, working remuneration, working environment, working interpersonal relationships, willingness to work and job promotion. 629 pieces of questionnaires were collected, among which, 612 were valid for analysis. The differences of job satisfaction between different levels of each influencing factor were tested by Analysis of Variance. Multiple linear stepwise regression was used to analyze the influencing factors of job satisfaction.   Results  The overall job satisfaction was mainly affected by income and position (β=0.133、0.114，P<0.05，R²=0.412). Meanwhile, the major influencing factors of the satisfaction of leadership behavior were age and income (β=－0.153、0.148，P<0.05，R²=0.300). The satisfaction of management system was mostly affected by the posts(β=0.115，P<0.05，R²=0.373). The satisfaction of remuneration and working environment were both mainly influenced by income and position (β=0.161、0.106，P<0.05，R²=0.404；β=0.198、0.133，P<0.05，R²=0.353). The major influencing factors of the satisfaction of job promotions were age and position (β=－0.177、0.093，P<0.05，R²=0.301).   Conclusion  TB institutions in Hunan should strengthen human resources construction, improve the staffing quantity and quality, improve HCWs income and establish a rational and fair distribution system.

Key words: Preventive health services, Health personnel, Job satisfaction, Hunan province