Clonging and expression of rv2653c gene of M.tuberculosis in E. coli

Abstract

Abstract: Objective To study the expression and purification of Rv2653c protein of Mycobacterium tuberculosis expressed in E.coli.Methods Rv2653c genes were amplified by polymerase chain reaction from genome of Mycobacterium tuberculosis H37Rv,and first cloned into vector pGEMT.After the sequence was confirmed,the gene was subcloned into vector pET24b.Recombinant Rv2653c protein was obtained by expression and purification.The antigenicity of the fused protein was analyzed by Western-blot.Results The DNA sequences of ...

Key words: Mycobacterium tuberculosis, Escherichia coli, bacterial proteins, recombinant proteins

CLC Number:

R378

LI Bang-Yin, SUN Wei-Guo, CHENG Xiao-Xing, SUN Chang-Wen, XIONG Zhi-Hong, WANG Zhong-Yuan, WANG Jin-He, SU Rui. Clonging and expression of rv2653c gene of M.tuberculosis in E. coli[J]. Chinese Journal of Antituberculosis, 2010, 32(11): 752-755.

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Clonging and expression of rv2653c gene of M.tuberculosis in E. coli

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