The application of mycobacterial interspersed repetitive unit(MIRU) in M.tuberculosis genotying

Abstract

Abstract: Objective To assess the application of mycobacterial interspersed repetitive unit(MIRU) in the epidemiology of tuberculosis, and to discuss its prospect. Method158 Mycobacterium tuberculosis isolates that had been typed by IS6110-RFLP were subjected to MIRU. ResultsThe Results were obtained by MIRU within 1 day. 105 patterns were produced, in which 65 patterns were unique. In MIRU loci, there were four loci(loci 4，10，26，40) with higher diversity. MIRU typing provided discrimination (Hunter-Gaston Index ［HGI］ of 0.978) compared with the IS6110 RFLP method (HGI=0.994). For those strains lower than 10 copies, MIRU typing provided discrimination (HGI=0.982) comparable to that of the RFLP method (HGI=0.98). ConclusionMIRU typing, which is simple, rapid, and easy to popularize, would exert important function in the epidemiological investigation of tuberculosis.

Key words: Mycobacterium tuberculosis, MIRU, IS6110, RFLP

Guo Yanling¹, Liu Yang，Wang Sumin, Li Chuanyou, Song Changxing. The application of mycobacterial interspersed repetitive unit(MIRU) in M.tuberculosis genotying[J]. Chinese Journal of Antituberculosis, 2009, 31(2): 80-84.

References

[1] 郭艳玲,王甦民,李传友,端木宏谨.以PCR为基础的结核分枝杆菌DNA分型技术及其应用[J]. 中国防痨杂志，2004，26（4）：250-254.
[2] 王甦民. 应重视结核病的实验诊断[J].中华检验医学杂志，2005,28(08): 769-770.
[3] Van Embden JD, Cave MD, Crawford JT, Dale JW, Eisenach KD, Gicquel B, Hermans P, Martin C, McAdam R, Shinnick TM. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology[J]. J Clin Microbiol，1993,31(2): 406-409.
[4] 郭艳玲,王甦民,李传友,刘洋.IS6110限制性片段长度多态性和间隔区寡核苷酸分型在结核分枝杆菌菌株鉴定中的应用[J]. 中华检验医学杂志，2005，28(08): 796-798.
[5] Arnold C. Molecular evolution of Mycobacterium tuberculosis[J]. Clin Microbiol Infect，2007,13(2):120-128.
[6] Supply P, Mazars E, Lesjean S, Vincent V, Gicquel B, Locht C. Variable human minisatellite-like regions in the Mycobacterium tuberculosis genome[J]. Mol Microbio, 2000,36(3): 762-771.
[7] Supply P，Lesjean S，Savine E, Kremer K, van Soolingen D, Locht C. Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on Mycobacterial interspersed repetitive units[J]. J Clin Microbiol, 2001,39(10): 3563-3571.
[8] Martín A, Herránz M, Serrano MJ, Bouza E, García de Viedma D. Rapid clonal analysis of recurrent tuberculosis by direct MIRU-VNTR typing on stored isolates[J]. BMC Microbiol, 2007,7:73.
[9] Oelemann MC, Diel R, Vatin V, Haas W, Rüsch-Gerdes S, Locht C, Nemann S, Supply P. Assessment of an optimized mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis[J]. J Clin Microbiol, 2007, 45(3):691-697.
[10] Gopaul KK, Brown TJ, Gibson AL, Yates MD, Drobniewski FA. Progression toward an improved DNA amplification-based typing technique in the study of mycobacterium tuberculosis epidemiology[J]. J Clin Microbiol, 2006,44(7): 2492-2498.
[11] 沈国妙,查佳,徐琳，孙斌，桂晓红，王奕峰，梅建，高谦.结核分枝杆菌散在分布重复单位基因型分型法的应用研究. 中华结核和呼吸杂志，2005， 28（5）：292-296.

[1]	Chinese Antituberculosis Association, Schools and Children Branch of the Chinese Antituberculosis Association, Editorial Board of Chinese Journal of Antituberculosis . Expert consensus of clinical application of the recombinant Mycobacterium tuberculosis fusion protein (EC) [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 761-768.
[2]	YANG Lei, WEI Fen, ZHANG Kai, QIU Jing-jing, WANG Ying-ying, DU Wei-xin, LU Jin-biao, TAO Li-feng, PU Jiang. Stability and efficacy study of recombinant Mycobacterium tuberculosis fusion protein (EC) [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 799-806.
[3]	ZHANG Kai, TAO Li-feng, WEI Fen, DU Wei-xin, QIU Jing-jing, CHEN Wei, CHEN Bao-wen, ZHU Yin-meng, CHENG Xing, SU Cheng, ZHONG Zai-xin, LU Jin-biao, PU Jiang. Immune characteristics and preclinical animal safety studies of recombinant Mycobacterium tuberculosis fusion protein(EC) [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 807-813.
[4]	ZHANG Kai, SHEN Xiao-bing, TAO Li-feng, WEI Fen, CHEN Bao-wen, QIU Jing-jing, CHEN Wei, LU Jin-biao, ZHU Yin-meng, CHENG Xing, ZHONG Zai-xin, ZHAO Ai-Hua, PU Jiang. Establishment of quality standards for recombinant Mycobacterium tuberculosis fusion protein [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 814-820.
[5]	DU Wei-xin, WEI Fen, LU Jin-biao, ZHAO Ai-hua, PU Jiang, WANG Guo-zhi, XU Miao. Preliminary establishment of national reference of the freeze-dried recombinant Mycobacterium tuberculosis allergen for the bulk potency evaluation [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 826-831.
[6]	QU Meng-jin, LIANG Zheng-min, WANG Yuan-zhi, ZHOU Xiang-mei. Research progress of carbohydrate metabolism of Mycobacterium tuberculosis [J]. Chinese Journal of Antituberculosis, 2020, 42(8): 874-879.
[7]	SONG Yi-meng, LI Yuan-chun, HE Wen-cong, HE Ping, BAO Jing-jing, LIU Chun-fa, LIU Dong-xin, WANG Xin-yang, ZHAO Yan-lin, LI Yan-ming. Cost analysis of fluorescence PCR probe melting curve technique for the detection of Mycobacterium tuberculosis resistance in China [J]. Chinese Journal of Antituberculosis, 2020, 42(7): 712-717.
[8]	ZHANG Jie, REN Yi-xuan, PAN Li-ping, ZHANG Zong-de. Application of whole genome sequencing in research of Mycobacterium tuberculosis [J]. Chinese Journal of Antituberculosis, 2020, 42(7): 737-740.
[9]	LI Xin-na, SHEN Xin-xin, WANG Rui-bai, DUAN Su-xia, ZHANG Rui-qing, WANG Rui-huan, BAI Xue-ding, FAN Guo-hao, WANG Jin-rong, GAO Yuan, CHEN Zi-wei, MA Xue-jun. Application value of probe-directed recombinase amplification assay in detecting MTB rpoB gene mutation [J]. Chinese Journal of Antituberculosis, 2020, 42(5): 481-488.
[10]	ZHANG Xing,WANG Feng-ming,LYU Xu-feng,HUA Tian-qi,ZHANG Xue-jun,JIANG Jing-yi,DING Chen-li,ZHU Wei,XIA Guo-dong,JI Jun-min,ZHAO Fei. Analysis of influencing factors of Mycobacterium tuberculosis infection in HIV/AIDS patients [J]. Chinese Journal of Antituberculosis, 2020, 42(4): 360-365.
[11]	ZHAO Jiao-jie,FU Lei,WANG Bin,ZHANG Lei,HU Ming-hao,LU Yu. Establishment and application of pharmacodynamics and pharmacokinetics model in vitro [J]. Chinese Journal of Antituberculosis, 2020, 42(4): 372-379.
[12]	YANG Han,YANG Jing-fen,WU Hao,CUI Xiao-li,DANG Li-yun. Value of MicroDST test in detecting sensitivity of first-line anti-tuberculosis drugs [J]. Chinese Journal of Antituberculosis, 2020, 42(4): 380-384.
[13]	CHEN Yan-mei,HUANG Xin-chun,SUN Qi,ZHANG Chen-chen,WEN Wen-pei,CHEN Liang. Analysis of drug resistance in patients with smear-negative and culture-positive pulmonary tuberculosis in Guangdong province in 2016 [J]. Chinese Journal of Antituberculosis, 2020, 42(3): 272-275.
[14]	SUN Jin-xia,ZHANG Qing-wen,LI Yin-hong,JIANG Xin. Study of autophagy induced by myricetin in MTB infected macrophages through PI3K/Akt/mTOR signaling pathway [J]. Chinese Journal of Antituberculosis, 2020, 42(2): 101-107.
[15]	LIU Bin-bin,GONG Dao-fang,CHEN Zhen-hua,GUO Jing-wei,YU Yan-yan,LIU Feng-ping,OU-Yang hui,TAN Yun-hong. Comparative study on detection rate and detection cost of Mycobacterium tuberculosis complex by three laboratory diagnostic techniques [J]. Chinese Journal of Antituberculosis, 2020, 42(2): 143-148.