Abstract: Objective The specific diagnosis for tuberculosis have more problems. To solve these problems , Im munoblots technique have be used . Methods Using Im munoblotstechniquefor detecting antibody that againstspecific antigen( T B S Ag 1) of M.tuberculosis. Results The Sensitivity was 89.5 % in 361 tuberculosis patients and the specificity was95.6 % in 150 nontuberculosis patients and 350 healthy controls . Making a comparison between Im munoblots and Myco Dottechnique . Conclusion Im munoblots technique have a higher positive rates . So suggested that Im munoblots technique for de tecting specificantibody of M.tuberculosis was a usefultoolfor diagnosis of tuberculosis .

Key words: Im m unoblots technique, Tuberculosis, Antibody