Pathogenic mechanism of Ag85 complex and its application progress in the development of tuberculosis vaccine

doi:10.3969/j.issn.1000-6621.2020.11.018

Abstract

Abstract:

Effective vaccines are urgently needed to control the transmission of Mycobacterium tuberculosis (MTB). A variety of MTB immunogenic molecules have been identified by now. And of them, the antigen 85 (Ag85) complex (Ag85A, Ag85B and Ag85C) is an important virulence factor, it could mediate the adhesion and invasion of MTB and the synthesis of the cell wall. Ag85 complex has been used in the construction of many new vaccines, such as recombinant attenuated vaccines, protein adjuvant vaccines and virus vector vaccines. In this paper, pathogenic mechanism of Ag85 complex and its application progress in the development of tuberculosis vaccine are reviewed, and the effects of Ag85 complex as antigens in developing different types of tuberculosis vaccines are also investigated.

Key words: Antigens,bacterial, Antigen-antibody complex, Virulence factors, Tuberculosis, Vaccines,synthetic, Review literature as topic

LIANG Zheng-min, WANG Yuan-zhi, LIU Yi-duo, ZHOU Xiang-mei. Pathogenic mechanism of Ag85 complex and its application progress in the development of tuberculosis vaccine[J]. Chinese Journal of Antituberculosis, 2020, 42(11): 1243-1249. doi: 10.3969/j.issn.1000-6621.2020.11.018

Figures/Tables 1

References 66

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疫苗类型及名称	结核抗原	临床试验情况	参考文献
重组BCG疫苗
rBCG30	过表达Ag85B	免疫原性不佳,Ⅰ期结束	[20]
AERAS-422	过表达产气荚膜溶血素O、Ag85B、Ag85A、Rv3407	有不良反应,Ⅰ期结束	[21]
rBCG::Ag85A	过表达Ag85A	下一代	[22]
rBCG-CMX	过表达Ag85C、MPT51、HspX	下一代	[23]
rBCG::XB	过表达Ag85B和HspX	下一代	[24]
病毒载体疫苗
MVA85A	牛痘病毒表达Ag85A	Ⅱ期	[19]
Ad35-TBS/AERAS-402	35型腺病毒表达Ag85A、Ag85B和TB10.4	Ⅰ期	[25]
Ad5Ag85A	5型腺病毒表达Ag85A	Ⅰ期	[26]
ChAdOx1.85 A	猴腺病毒表达Ag85A	Ⅰ期	[27]
Ad5-gsgAM	5型腺病毒表达Ag85A和Mtb32 (Rv0125)	下一代	[28]
SeV85AB	仙台病毒表达Ag85A和Ag85B	下一代	[29]
蛋白佐剂疫苗
H1∶IC31	Ag85B-ESAT-6,佐剂:IC31	Ⅱ期	[30]
H4∶IC31	Ag85B-TB10.4,佐剂:IC31	Ⅱ期	[31]
H56∶IC31	Ag85B-ESAT-6 和Rv2660,佐剂:IC31	Ⅰ期	[32]
EAMM	ESAT-6-Ag85B-MPT6-Mtb8.4,佐剂:DDA,poly I∶C	下一代	[33]
Ag85B-HspX	Ag85B-HspX,佐剂:AG,poly I:C	下一代	[34]
GamTBvac	ESAT6-CFP10和Ag85A,佐剂:Dextrans,CpG ODN	Ⅰ期	[11]
Ag85B∶hfcγ1	融合蛋白Ag85B-hfcγ1	下一代	[35]
DNA疫苗
HG856A	表达Ag85A、ESAT-6	下一代	[36]
A39	表达Ag85A、Rv3425和Rv2029c	下一代	[37]
pAEH	表达Ag85B-ESAT-6-HspX的	下一代	[38]
DNA-Mkan85B	表达Ag85B	下一代	[39]