Chinese Journal of Antituberculosis ›› 2019, Vol. 41 ›› Issue (6): 609-615.doi: 10.3969/j.issn.1000-6621.2019.06.005
• Original Articles • Previous Articles Next Articles
Zhao-gang SUN(),Hong-jing ZHANG,Zi-hui LI,Qi SUN,Lin-na LYU,Li-ping PAN,Gilbert Sandy,Zong-de ZHANG,Shao-fa XU,Xia James()
Received:
2019-03-20
Online:
2019-06-10
Published:
2019-06-04
Contact:
Zhao-gang SUN,Xia James
E-mail:sunzg75@163.com;james.xia@genosensorcorp.com
Zhao-gang SUN,Hong-jing ZHANG,Zi-hui LI,Qi SUN,Lin-na LYU,Li-ping PAN,Gilbert Sandy,Zong-de ZHANG,Shao-fa XU,Xia James. Design of a next generation microsequencing gene microarray and preliminary study of its effect on drug resistance detection[J]. Chinese Journal of Antituberculosis, 2019, 41(6): 609-615. doi: 10.3969/j.issn.1000-6621.2019.06.005
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URL: http://www.zgflzz.cn/EN/10.3969/j.issn.1000-6621.2019.06.005
基因位点 | 碱基突变形式 | 菌株数(株) | 突变频率(%) |
---|---|---|---|
inhA基因 | |||
-8(T) | C | 6 | 5.50 |
-15(C) | T | 24 | 22.02 |
katG基因 | |||
315(AGC) | ACC | 68 | 62.39 |
315(AGC) | AAC | 2 | 1.83 |
rrs基因 | |||
1401(A) | G | 14 | 12.84 |
1402(C) | T | 2 | 1.83 |
eis基因 | |||
-10(G) | A | 3 | 2.75 |
-14(C)a | T | 1 | 0.92 |
-37(G) | T | 2 | 1.83 |
rpoB基因 | |||
511(CTG) | CCG | 4 | 3.67 |
513(CAA) | AAA | 1 | 0.92 |
516(GAC) | GTC | 5 | 4.59 |
526(CAC) | CTC | 5 | 4.59 |
526(CAC) | TAC | 6 | 5.50 |
526(CAC) | GAC | 4 | 3.67 |
526(CAC) | CGC | 4 | 3.67 |
531(TCG) | TAC | 1 | 0.92 |
531(TCG) | TGG | 4 | 3.67 |
531(TCG) | TTG | 63 | 57.80 |
rpsL基因 | |||
43(AAG) | AGG | 37 | 33.94 |
88(AAG) | AGG | 16 | 14.68 |
88(AAG) | CAG | 2 | 1.83 |
gyrA基因 | |||
90(GCG) | GTG | 18 | 16.51 |
91(TCG) | CCG | 4 | 3.67 |
94(GAC)a | AAC | 5 | 4.59 |
94(GAC) | CAC | 2 | 1.83 |
94(GAC) | GCC | 18 | 16.51 |
94(GAC) | GGC | 34 | 31.19 |
94(GAC) | TAC | 2 | 1.83 |
94(GAC)a | TTC | 2 | 1.83 |
embB基因 | |||
306(ATG) | ATA | 13 | 11.93 |
306(ATG) | ATC | 2 | 1.83 |
基因位点 | 碱基突变形式 | 菌株数(株) | 突变频率(%) |
306(ATG) | ATT | 1 | 0.92 |
306(ATG) | CTG | 1 | 0.92 |
306(ATG) | GTA | 35 | 32.11 |
306(ATG)a | GTC | 2 | 1.83 |
306(ATG) | GTG | 36 | 33.03 |
306(ATG)a | TTA | 1 | 0.92 |
306(ATG) | TTG | 1 | 0.92 |
406(GGC)a | AGC | 7 | 6.42 |
406(GGC) | GAC | 8 | 7.34 |
406(GGC) | GCC | 8 | 7.34 |
497(CAG) | CGG | 3 | 2.75 |
探针名称 | 检测结果 | 符合数 (株) | 符合率 (%) | 探针名称 | 检测结果 | 符合数 (株) | 符合率 (%) | 探针名称 | 检测结果 | 符合数 (株) | 符合率 (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
inhA_-8_S_T | 2株假阴性 | 107 | 98.17 | rpoB_1303-4_A_TC | 1株假阳性 | 108 | 99.08 | gyrA_269_S_T | 全部正确 | 109 | 100.00 |
inhA_-8_A_G | 全部正确 | 109 | 100.00 | rpoB_1304_A_A | 2株假阴性 | 107 | 98.17 | gyrA_271_A_G_2nd | 2株假阴性 | 107 | 98.17 |
inhA_-15_A_A | 1株假阴性, 2株假阳性 | 106 | 97.25 | rpoB_1304_A_Ca | 全部正确 | 109 | 100.00 | gyrA_280-1_S_CA | 全部正确 | 109 | 100.00 |
katG_944_S_A | 全部正确 | 109 | 100.00 | rpoB_1304_A_Ga | 全部正确 | 109 | 100.00 | gyrA_280-1_S_TA | 全部正确 | 109 | 100.00 |
katG_944_S_C | 2株假阴性, 3株假阳性 | 104 | 95.41 | rpoB_1333-4_A_GGa | 全部正确 | 109 | 100.00 | gyrA_280-1_S_GC | 全部正确 | 109 | 100.00 |
katG_944-5_A_TCa | 全部正确 | 109 | 100.00 | rpoB_1333-4_A_TAa | 1株假阳性 | 108 | 99.08 | gyrA_280-1_S_GG | 全部正确 | 109 | 100.00 |
katG_945_A_Ca | 全部正确 | 109 | 100.00 | rpoB_1333-4_A_TC | 1株假阴性 | 108 | 99.08 | embB_916-8_A_CAT | 3株假阴性, 31株假阳性 | 75 | 68.81 |
katG_945_A_Ta | 全部正确 | 109 | 100.00 | rpoB_1333-4_S_CA | 1株假阴性 | 108 | 99.08 | embB_916-8_S_GTA | 全部正确 | 109 | 100.00 |
rrs1401_S_A | 全部正确 | 109 | 100.00 | rpoB_1334_A_A | 全部正确 | 109 | 100.00 | embB_916-8_S_CTG | 全部正确 | 109 | 100.00 |
rrs1401_S_G | 全部正确 | 109 | 100.00 | rpoB_1348-50_S_TAGa | 全部正确 | 109 | 100.00 | embB_916-8_S_ATA | 2株检测 假阴性 | 107 | 98.17 |
rrs1401_S_Ta | 全部正确 | 109 | 100.00 | rpoB_1348-50_S_TAC | 全部正确 | 109 | 100.00 | embB_916-8_S_ATT | 1株检测 假阴性 | 108 | 99.08 |
rrs1402_A_G | 1株假阳性 | 108 | 99.08 | rpoB_1348-50_A_CGA | 3株假阴性 | 106 | 97.25 | embB_916-8_S_ATC | 1株检测 假阴性 | 108 | 99.08 |
rrs1401_1402_S_AT | 全部正确 | 109 | 100.00 | rpoB_1348-50_A_AGAa | 全部正确 | 109 | 100.00 | embB_916-8_S_GTG | 2株假阴性, 3株假阳性 | 104 | 95.41 |
探针名称 | 检测结果 | 符合数 (株) | 符合率 (%) | 探针名称 | 检测结果 | 符合数 (株) | 符合率 (%) | 探针名称 | 检测结果 | 符合数 (株) | 符合率 (%) |
rrs1484_S_Ta | 全部正确 | 109 | 100.00 | rpoB_1348-50_S_TGG | 全部正确 | 109 | 100.00 | embB_916-8_S_TTG | 1株检测 假阴性 | 108 | 99.08 |
rrs1484_A_Ca | 全部正确 | 109 | 100.00 | rpoB_1348-50_S_TTG | 1株假阳性, 1株假阴性 | 107 | 98.17 | embB_1216-8_A_TCG | 全部正确 | 109 | 100.00 |
eis_-10_S_G | 1株未检出 | 108 | 99.08 | rpoB_1355_A_A_a | 全部正确 | 109 | 100.00 | embB_1216-8_A_GCC | 1株检测 假阳性 | 108 | 99.08 |
eis_-37_A_C | 全部正确 | 109 | 100.00 | rpsL_128_A_C | 2株假阴性 | 107 | 98.17 | embB_1216-8_A_GGC | 1株假阴性, 4株错误 | 104 | 95.41 |
rpoB_1289_S_C | 全部正确 | 109 | 100.00 | rpsL_128_S_A | 全部正确 | 109 | 100.00 | embB_1216-8_A_GTC | 5株假阴性 | 104 | 95.41 |
rpoB_1289_S_T | 全部正确 | 109 | 100.00 | rpsL_128_S_Ca | 全部正确 | 109 | 100.00 | embB_1489-90_S_AA | 全部正确 | 109 | 100.00 |
rpoB_1294-5_S_AA | 全部正确 | 109 | 100.00 | rpsL_262-3_S_AA | 全部正确 | 109 | 100.00 | embB_1489-90_S_CA | 17株假阴性 | 92 | 84.40 |
rpoB_1294-5_S_CA | 全部正确 | 109 | 100.00 | rpsL_262-3_S_ACa | 全部正确 | 109 | 100.00 | embB_1489-90_S_CG | 1株检测 假阴性 | 108 | 99.08 |
rpoB_1294-5_S_CG | 全部正确 | 109 | 100.00 | rpsL_262-3_S_AG | 全部正确 | 109 | 100.00 | ||||
rpoB_1303-4_A_TA | 全部正确 | 109 | 100.00 | rpsL_262-3_S_CA | 全部正确 | 109 | 100.00 |
基因芯片 检测 | 基因测序 | 敏感度 (%) | 特异度 (%) | 阳性预测 值(%) | 阴性预测 值(%) | 一致率 (%) | Kappa值 | |
---|---|---|---|---|---|---|---|---|
突变(株) | 未突变(株) | |||||||
embB基因 | 64.21 (53.66~3.60) | 92.86 (64.17~99.63) | 98.39 (90.17~99.92) | 27.66 (16.09~42.87) | 67.89 (58.62~75.94) | 0.29 | ||
突变 | 61 | 1 | ||||||
未突变 | 34 | 13 | ||||||
gyrA基因 | 80.00 (44.22~96.46) | 100.00 (95.35~100.00) | 100.00 (59.77~100.00) | 98.02 (92.34~99.66) | 98.17 (93.15~99.90) | 0.88 | ||
突变 | 8 | 0 | ||||||
未突变 | 2 | 99 | ||||||
inhA基因 | 95.83 (76.88~99.78) | 97.65 (90.96~99.59) | 92.00 (72.5~98.60) | 98.81 (92.63~99.94) | 97.25 (91.87~99.41) | 0.92 | ||
突变 | 23 | 2 | ||||||
未突变 | 1 | 83 | ||||||
katG基因 | 98.55 (91.11~99.92) | 87.50 (72.40~95.31) | 93.15 (84.07~97.45) | 97.22 (83.80~99.85) | 94.50 (88.27~97.69) | 0.88 | ||
突变 | 68 | 5 | ||||||
未突变 | 1 | 35 | ||||||
rpoB基因 | 92.63 (84.91~96.73) | 85.71 (56.15~97.49) | 97.78 (91.44~99.61) | 63.16 (38.63~82.77) | 91.74 (84.87~95.78) | 0.68 | ||
突变 | 88 | 2 | ||||||
未突变 | 7 | 12 | ||||||
rpsL基因 | 93.85 (84.21~98.01) | 63.64 (47.74~77.17) | 79.22 (68.17~87.31) | 87.50 (70.07~95.92) | 81.65 (73.27~87.87) | 0.60 | ||
突变 | 61 | 16 | ||||||
未突变 | 4 | 28 | ||||||
eis基因 | 75.00 (21.94~98.68) | 100.00 (95.60~100.00) | 100.00 (31.00~100.00) | 99.06 (94.10~99.95) | 99.08 (94.48~99.99) | 0.85 | ||
突变 | 3 | 0 | ||||||
未突变 | 1 | 105 | ||||||
rrs基因 | 94.44 (70.63~99.71) | 98.90 (93.17~99.94) | 94.44 (70.63~99.71) | 98.90 (93.17~99.94) | 98.17 (93.15~99.90) | 0.93 | ||
突变 | 17 | 1 | ||||||
未突变 | 1 | 90 |
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