Clinical value of automatic fluorescence mycob.T stainer and scanner for detecting tuberculosis

doi:10.3969/j.issn.1000-6621.2018.10.009

Abstract

Abstract:

Objective To observe the clinic value of automatic fluorescence Mycob.T Stainer and Scanner (AFSS) for detecting tuberculosis. Methods A total of 2243 sputum specimens from 748 suspected TB patients were tested at Linyi People’s Hospital from September to December in 2016. Each specimen was subjected to 2 direct smear slides. One slide was stained by fluorescence method and read with conventional fluorescence microscopy (FM) manually. Another slide was stained and scanned by AFSS. All the specimens were decontaminated by 4% NaOH and then inoculated into Lowenstein-Jensen (L-J) media for comparison. The performance of AFSS was analyzed compared with culture. All discrepancy slides between AFSS and conventional FM were rechecked by Ziehl-Neelsen microscopy. Results The average positive rate of AFSS was 32.1% while the average positive rate of conventional FM was 31.92%. No significant difference was observed (χ ²=0.016,P>0.05). Compared to culture, the sensitivity and specificity of AFSS for detecting Mycobacteria was 83.4% and 95.9%, respectively. The positive predictive value and negative predictive value was 91.6% and 91.4%, respectively. Conclusion AFSS can detect Tuberculosis with high sensitivity, specificity and automation, and deserve to be promoted.

Key words: Mycobacterium tuberculosis, Microscopy, Fluorescence, Automation, Comparative study

Qiang LI,Hong-guo YANG,Yun-feng DENG,Xi-chao OU,Hui XIA,Yan-lin ZHAO. Clinical value of automatic fluorescence mycob.T stainer and scanner for detecting tuberculosis[J]. Chinese Journal of Antituberculosis, 2018, 40(10): 1075-1078. doi: 10.3969/j.issn.1000-6621.2018.10.009

Figures/Tables 4

References 14

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检测技术	检测阳性份数	阳性率(%)	χ²值	P值
AFSS	720	32.1	0.016	0.898
传统FM	716	31.9

AFSS检测结果	传统 FM检测结果
AFSS检测结果	实际条数	+	++	+++	++++	阴性	合计
实际条数	11	46	8	6	0	16	87
+	8	97	48	44	5	16	218
++	4	42	45	58	18	3	170
+++	0	13	37	56	38	0	144
+++	0	4	13	33	51	0	101
阴性	3	11	12	4	1	1492	1523
合计	16	239	163	201	113	1527	2243

AFSS及传统FM检测结果	培养结果
AFSS及传统FM检测结果	阳性	阴性	合计
AFSS阳性, FM阴性	19	16^a	35
AFSS阴性, FM 阳性	30^b	1	31
合计	49	17	66

检测方法结果	培养阳性 (份)	培养阴性 (份)	敏感度 (%)	特异度 (%)	阳性预测值 (%)	阴性预测值 (%)
AFSS
阳性	657	60	83.4	95.9	91.6	91.4
阴性	131	1386	(80.8~86.0)	(94.8~96.9)	(89.6~93.7)	(90.0~92.8)
传统FM
阳性	668	45	84.8	96.9	93.7	92.1
阴性	120	1401	(82.3~87.3)	(96.0~97.8)	(81.9~95.5)	(80.8~93.5)

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