Differential expression and diagnostic value of three cytokines in pleural effusion between pulmonary tuberculosis and lung cancer

doi:10.3969/j.issn.1000-6621.2018.10.004

Abstract

Abstract:

Objective To analyze the difference of macrophage migration inhibitory factor (MIF), retinoid acid related orphan receptor alpha (RORα) and retinoid acid related orphan receptor gamma (RORγ) in the pleural effusion of patients with pulmonary tuberculosis (TB) and lung cancer, and to explore their values in the differential diagnosis. Methods By simple random sampling, the frozen pleural effusion samples of pulmonary tuberculosis and lung cancer were numbered, and 80 samples were collected, the basic information of the subjects was summarized. The levels of MIF, RORα and RORγ in the pleural effusion were detected by enzyme-linked immunosorbent assay (ELISA). The application software SPSS 20.0 was used for comparison and analysis, and the measurement data in this study do not conform to normal distribution, so the Mann-Whitney U test has been used, the difference was statistically significant when P<0.05. Results The medians of MIF level (quartile) [M (Q₁,Q₃)] in the pleural effusion of the tuberculosis group and lung cancer group were 5.83 (2.41, 16.43) and 2.79 (0.91, 11.12) ng/L respectively, there was significant difference between the groups (U=2314.50,P<0.01). The RORα levels [M (Q₁, Q₃)] in the pleural effusion of the tuberculosis group and lung cancer group were 0.63 (0.37, 1.66) and 0.63 (0.57, 2.16) ng/L respectively, there was no significant difference between the two groups (U=3525.00, P>0.05); the levels of RORγ in the pleural effusion of the tuberculosis group and lung cancer group were 3.23 (1.82, 5.26) and 3.44 (1.94, 7.11) ng/L respectively, with no significant difference (U=3431.50, P>0.05). Conclusion The level of MIF in the pleural effusion of patients with tuberculosis is higher than the patients with lung cance, the detection of MIF level in pleural effusion is certainly diagnostically valuable in differentiating pulmonary tuberculosis from lung cancer. And the diagnostic value of RORα and RORγ in pleural effusion remains to be verified.

Key words: Tuberculosis, pulmonary, Lung neoplasms, Pleural effusion, Macrophage migration-inhibitory factors, Orphan nuclear receptors, Enzyme-linked immunosorbent assay, Diagnosis, differential

Hao-ran LIU,Ya-li ZHANG,Wei-cong REN,Ling-juan ZHAO,Chuan-you LI,Wei WANG,Meng-qiu GAO. Differential expression and diagnostic value of three cytokines in pleural effusion between pulmonary tuberculosis and lung cancer[J]. Chinese Journal of Antituberculosis, 2018, 40(10): 1046-1050. doi: 10.3969/j.issn.1000-6621.2018.10.004

Figures/Tables 4

References 15

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一般特征	肺结核组	肺癌组	合计
性别
男	51(63.75)	59(73.80)	110(68.75)
女	29(36.25)	21(26.30)	50(31.25)
年龄(岁)
<30	21(26.25)	1(1.25)	22(13.75)
30~	38(47.50)	34(42.50)	72(45.00)
>60	21(26.25)	45(56.25)	66(41.25)

细胞因子组别	中位数(M)(ng/L)	四分位数(Q₁,Q₃)(ng/L)	U值	P值
MIF			2314.50	0.003
肺结核组	5.83	2.41,16.43
肺癌组	2.79	0.91,11.12
RORα			3525.00	0.267
肺结核组	0.63	0.37,1.66
肺癌组	0.63	0.57,2.16
RORγ			3431.50	0.430
肺结核组	3.23	1.82,5.26
肺癌组	3.44	1.94,7.11