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Chinese Journal of Antituberculosis ›› 2013, Vol. 35 ›› Issue (1): 13-16.

Special Issue: GeneXpert MTB/RIF检测技术相关研究

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Study on Mycobacteria tuberculosis and rifampin resistance detection of Xpert Mtb/RIF test

ZHANG Zhi-guo, OU Xi-chao, SUN Qian, DU Chun-ying, GAO Tie-jie, ZHAO Yan-lin   

  1. Clinical laboratory, Tuberculosis Dispensary of Changping District, Beijing 102200, China
  • Received:2012-11-18 Online:2013-01-10 Published:2013-04-03
  • Contact: ZHAO Yan-lin E-mail:zhaoyanlin@chinatb.org

Abstract: Objective  To evaluate the performance of Xpert Mtb/RIF test on detecting tuberculosis and rifampin resistance for patients.   Methods  Recruited 588 initial diagnosis patients, selected sputum samples to do smear, solid culture, traditional proportion method drug susceptibility test and Xpert Mtb/RIF test. Analyze the sensitivity and specificity of Xpert Mtb/RIF method in detecting tuberculosis and refampin resistance.  Results  According to the solid culture result, the sensitivity and specificity of Xpert Mtb/RIF test in detecting tuberculosis are 95.0% (345/363) and 89.6% (147/164). According to the conventional drug susceptibility test result, the sensitivity and specificity in detecting rifampin resistance are 89.1% (41/46) and 96.3% (289/300) respectively.  Conclusion  The Xpert Mtb/RIF test has very high sensitivity and specificity. It has very good application prospects in rapidly detection tuberculosis and rifampin resistance.

Key words: Mycobacterium tuberculosis, Rifampin, Drug resistance, bacterial, Nucleic acid amplification techniques, Sensitivity and specificity