Analyses of 23S rRNAs of 39 clarithomycin-resistant  Mycobacterium tuberculosis clinical isolates

Abstract

Abstract: Objective To investigate the changes of 23S rRNA at site 2058 of clarithromycin-resistant Mycobacterium tuberculosis clinical isolates. Methods One Mycobacterium tuberculosis H37Rv standard strain and 64 isolates were detected, in which 39 clarithromycin-resistant isolates, 25 clarithromycin-sensitive isolates. The 23S rRNAs of Mycobacterium tuberculosis isolates were analyzed by polymerase chain reaction (PCR) and sequencing. Results The 23S rRNA of Mycobacterium tuberculosis H37Rv standard strain did not found the mutation at site 2058. Of 64 Mycobacterium tuberculosis isolates, only 1 extensively drug-resistant (XDR) strain had A2058A-G mutation of 23S rRNA, which account 2.56%（1/39） clarithromycin-resistant strains and 1/10 XDR strains. Conclusion The mutation of 23S rRNA at site 2058 of Mycobacterium tuberculosis may not be the main cause inducing its clarithromycin resistance. The mechanism of Mycobacterium tuberculosis clarithromycin resistance need be further studied.

Key words: Mycobacterium tuberculosis, Clarithromycin, RNA, ribosomal, 23S, Drug resistance, bacterial

KUANG Xiao-jia, TAN Shou-yong, WU Bi-tong, CAI Xing-shan, ZHANG Yuan-liang. Analyses of 23S rRNAs of 39 clarithomycin-resistant Mycobacterium tuberculosis clinical isolates[J]. Chinese Journal of Antituberculosis, 2013, 35(9): 690-692.

References

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Analyses of 23S rRNAs of 39 clarithomycin-resistant Mycobacterium tuberculosis clinical isolates

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